Bi7030c Bacterial physiology - practical course

Faculty of Science
Autumn 2020
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. Ivan Kushkevych, Ph.D. (seminar tutor)
Mgr. Martin Struk, Ph.D. (assistant)
Guaranteed by
doc. Ivan Kushkevych, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi7030c/01: Wed 13:00–16:50 E25/111, I. Kushkevych
Bi7030c/02: Thu 14:00–17:50 E25/111, I. Kushkevych
Prerequisites
Examination - organic chemistry, biochemistry, microbiology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 16 student(s).
Current registration and enrolment status: enrolled: 0/16, only registered: 0/16, only registered with preference (fields directly associated with the programme): 0/16
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand principles of work with bacterial and yeasts cells and bacteriophages and to understand chosen tasks, to solve various problems they can face in bacterial physiology; to use set of chosen techniques used in physiological laboratories; to work with growth characteristics of bacterial cells and phages, to understand influence of temperature and UV on lysogenic cells and phages; to prepare crude enzymatic preparation and to test its enzymatic activity and test physiological properties of industrial strains of yeasts. Students will learn to evaluate experimental data acquired, to interpret these results and to deduce conclusions from them. In case of unexpected results they will try to identify possible mistakes/problems. Students will produce a written report from acquired results as preparation for publication in scientific journals.
Learning outcomes
Student will be able - to characterize conditions for determination of physiological properties of bacteria - to determine the bacterial cell phagotype - compare the enzymatic activity of native cells and enzyme preparations - to determine the impact of environmental factors on the viability and vitality of microorganisms
Syllabus
  • Determination of bacterial proteins and dry-weight. Bacterial growth curve in static culture (using plate counts). One-step growth curve of polyvalent and virulent staphylopage. Release of bacteriophage from lysogenic culture by UV induction. Heat inactivation of a bacteriophage. Determination of dehydrogenase activity. Preparation of crude enzymatic product(HEP) with phosphatase activity. Determination of phosphatase activity of native cells and HEP. Influence of heavy metals on bacterial growth (Bioscreen)and protein production. Flocculation of yeasts.
Literature
  • Schemata pro jednotlivé úlohy poskytne vyučující. Laboratory protocols are provided by teacher.
  • Horáková, D., Němec, M. (2003) : Laboratorní cvičení z fyziologie bakterií (Laboratory protocols from bacterial physiology)
Teaching methods
Topic of each practical is at first explained and demonstrated to students. Then students are solving the practical task by themselves. The outcome of each practical is written report.
Assessment methods
Active participation at practical course, reports, credit.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2020, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2020/Bi7030c