C9531 Structure of biomacromolecules

Faculty of Science
Autumn 2013
Extent and Intensity
0/1/0. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
Mgr. Milan Zachrdla, Ph.D. (seminar tutor), prof. Mgr. Lukáš Žídek, Ph.D. (deputy)
Mgr. Pavel Srb, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Lukáš Žídek, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: prof. Mgr. Lukáš Žídek, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Timetable of Seminar Groups
C9531/01: Tue 12:00–12:50 C04/118
C9531/02: Thu 9:00–9:50 C04/118
C9531/03: Thu 10:00–10:50 C04/118
C9531/04: Tue 10:00–10:50 C04/118
Prerequisites
NOW( c9530 Structure of biomacromolecules )
The seminar is offered to students attending course C9530 (Structure of biomacromolecules).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the seminar is to offer the chance to practice the knowledge acquired in course C9530 (Structure of biomacromolecules) and to prepare students for the exam of the course C9530. Students attending the course will be able to analyze molecular structures, solving problems modeled as real examples from the laboratory practice, and combine results of various types of analyses to obtain a complex solution.
Syllabus
  • 1. Torsion angle determination, work with protein models. 2. Work with nucleic acid models, determination of pseudorotation. 3. Program Rasmol, demonstration of molecular mechanics. 4. Nucleic acid sequencing, designing site-directed mutagenesis. 5. Peptide sequencing using mass spectrometry. 6. IR and CD protein spectra interpretation. 7. Sequential assignment of protein NMR spectra. 8. Sequential assignment of nucleic acid NMR spectra. 9. X-ray diffraction - resolution, phase problem, refinement. 10. Work with databases of sequences and structures.
Literature
  • LESK, Arthur M. Introduction to protein architecture : the structural biology of proteins. Oxford: Oxford University Press, 2001, xii, 347. ISBN 0198504748. info
  • CAVANAGH, John. Protein NMR spectroscopy : principles and practice. 2nd ed. Amsterdam: Elsevier, 2007, xxv, 885. ISBN 9780121644918. info
Teaching methods
Practical problems are presented during the semester. After a detailed analysis of the model solution students demonstrate their ability to solve the problems individually in a form of tests.
Assessment methods
Students are required to solve at least 60% problems in each of oral test during semester. Excellent performance will be taken into account during the C9530 examination.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Teacher's information
http://www.ncbr.chemi.muni.cz/~lzidek/C9531/C9531.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2013, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2013/C9531