S1004 Methods for structural characterization of biomolecules

Faculty of Science
Autumn 2023
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
Mgr. Josef Houser, Ph.D. (lecturer)
Mgr. Filip Melicher (lecturer)
MVDr. Eva Paulenová, Ph.D. (lecturer)
prof. RNDr. Michaela Wimmerová, Ph.D. (lecturer)
Guaranteed by
Mgr. Josef Houser, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
At the end of the course students should be able to:
characterize protein sample for structural studies
understand and explain principles of crystal structure determination of proteins
solve X-ray protein structure
interpret electron density maps, analyze and validate pdb coordinates
Learning outcomes
At the end of the course students should be able to:
characterize protein sample for structural studies
understand and explain principles of crystal structure determination of proteins
solve X-ray protein structure
interpret electron density maps, analyze and validate pdb coordinates
Syllabus
  • 1. Biomolecular sample, characteristics, sample requirements 2. Sample quality analysis – stability, homogeneity 3. Structural methods for analysis of biomolecules, complementarity of techniques 4. Biomacromolecular crystals, crystallization techniques, crystallization experiment 5. Crystal properties, space group and symmetry, assymetric unit 6. Diffraction experiment, diffraciton images processing, methods for phasing, electron density 7. Structural model, density maps, model accuracy, validation, structural databases 8. Optimization of structural experiment 9. Laboratory practicals – characterization of protein sample and crystallization experiments
Literature
    recommended literature
  • PECORA R. Dynamic Light Scattering: Applications of Photon Correlation Spectroscopy, Springer, 1985
  • Bergfors, T. M. Protein Crystallization: Second Edition, Oxford University Press, 2009.
  • Fundamentals of crystallography. Edited by Carmelo Giacovazzo. 2nd ed. Oxford: Oxford University Press, 2002, xix, 825 s. ISBN 0-19-850957-X. info
  • Circular dichroism and the conformational analysis of biomolecules. Edited by Gerald D. Fasman. New York: Plenum Press, 1996, ix, 738 s. ISBN 0-306-45142-5. info
Teaching methods
Intensive course covering lectures and class discussion
Assessment methods
in-term assessment, final written test
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
Note related to how often the course is taught: during exam period, February 7-9, 2017.
Teacher's information
Course is being taught as a block of lectures, discussions and laboratory exercises at the end of semester - in the examination period.
The course is also listed under the following terms Autumn 2011 - acreditation, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2024.
  • Enrolment Statistics (Autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2023/S1004