Bi6270c Cytogenetics - practice

Faculty of Science
Spring 2005
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Petr Kuglík, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Timetable
Tue 16:00–19:50 Bpr,02004
Prerequisites
NOW( Bi6270 Cytogenetics )
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Cytogenetics is one of the oldest branches of genetics. The course is targeted at undergraduate students who need a general introduction to cytogenetics. The two main goals are followed: To present to students detailing all aspect of chromosome biology and the practical teaching of modern cytogenetic techniques and methods.
Syllabus
  • 1.Technical equipment of cytogenetic laboratory. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. 2. Basic techniques of cytogenetic preparation making. Plant cytogenetics. Pretreatment, fixation, maceration, Cytochemical and histochemical staining methods, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Human cytogenetics. Chromosome identification, karyotyp, idiogram. Chromosome banding methods. Q, G, C, R, N-banding. Chromosomal aberrations. Cytogenetic abnormalities in hematological malignancies and solid tumors. An International System for Human Cytogenetic Nomenclature (ISCN). 4. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. 5. Application of computer image analysis in human cytogenetics.
Literature
  • Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
  • MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
  • Chromosome analysis protocols. Edited by John R. Gosden. Totowa: Humana Press, 1994, xi, 508 s. ISBN 0-89603-243-4. info
Assessment methods (in Czech)
Praktická cvičení. Zápočet.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2005, recent)
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