PřF:Bi6270c Cytogenetics - pr. - Course Information
Bi6270c Cytogenetics - practice
Faculty of ScienceSpring 2008 - for the purpose of the accreditation
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (seminar tutor)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Prerequisites
- NOW( Bi6270 Cytogenetics )
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- Cytogenetics is one of the oldest branches of genetics. The course is targeted at undergraduate students who need a general introduction to cytogenetics. The two main goals are followed: To present to students detailing all aspect of chromosome biology and the practical teaching of modern cytogenetic techniques and methods.
- Syllabus
- 1.Technical equipment of cytogenetic laboratory. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. 2. Basic techniques of cytogenetic preparation making. Plant cytogenetics. Pretreatment, fixation, maceration, Cytochemical and histochemical staining methods, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Human cytogenetics. Chromosome identification, karyotyp, idiogram. Chromosome banding methods. Q, G, C, R, N-banding. Chromosomal aberrations. Cytogenetic abnormalities in hematological malignancies and solid tumors. An International System for Human Cytogenetic Nomenclature (ISCN). 4. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. 5. Application of computer image analysis in human cytogenetics.
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Chromosome analysis protocols. Edited by John R. Gosden. Totowa: Humana Press, 1994, xi, 508 s. ISBN 0-89603-243-4. info
- Assessment methods (in Czech)
- Praktická cvičení. Zápočet.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: every week.
- Enrolment Statistics (Spring 2008 - for the purpose of the accreditation, recent)
- Permalink: https://is.muni.cz/course/sci/spring2008-forthepurposeoftheaccreditation/Bi6270c