C8980c Protein Preparation and Characterization I - practice

Faculty of Science
Spring 2020
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
RNDr. Lubomír Janda, Ph.D. (lecturer)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: RNDr. Lubomír Janda, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites
C8980 Protein preparation || NOW( C8980 Protein preparation )
Basic knowledge of biochemistry and/or molecular biology is prerequisite for registration for the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 19 fields of study the course is directly associated with, display
Course objectives
Students completing the practical course will be able to use expression system for protein production, select suitable purification method and to analyze obtained results.
Learning outcomes
Upon completion of the laboratory course, students are able to: - design a cloning strategy - express protein in a heterologous prokaryotic system - purify protein (protein precipitation, affinity, ion-exchange and size-exclusion chromatography) - analyse protein function and purity.
Syllabus
  • 1. Bioinformatics and theory of DNA cloning. 2. Protein expression in bacteria. 3. Cell desintegration. 4. Purification. 5. SDS-PAGE. 6. protein activity measurement.
Literature
    recommended literature
  • HARDIN, Charles. Cloning, gene expression and protein purification : experimental procedures and process rationale. New York: Oxford University Press, 2001, viii, 435. ISBN 0195132947. info
  • Guide to protein purification. Edited by Richard R. Burgess - Murray P. Deutscher. 2nd ed. Boston: Elsevier/Academic Press, 2009, liii, 851. ISBN 9780123749789. info
Teaching methods
Practical course
Assessment methods
Laboratory protocol, results presentation
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: blok 5 dnů.
The course is also listed under the following terms Autumn 2012, Autumn 2013, Autumn 2014, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2020, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2020/C8980c