aVLBC0422c Biochemie II - cvičení

Lékařská fakulta
jaro 2020
Rozsah
0/3/0. 45. 2 kr. Ukončení: z.
Vyučující
doc. RNDr. Jiří Dostál, CSc. (cvičící)
Mgr. Jana Gregorová, Ph.D. (cvičící)
MUDr. Miroslava Hlaváčová, Ph.D. (cvičící)
PharmDr. Katarína Kostolanská (cvičící)
MUDr. Michaela Králíková, Ph.D. (cvičící)
RNDr. Hana Paulová, CSc. (cvičící)
doc. Mgr. Ondřej Peš, Ph.D. (cvičící)
Mgr. Jiří Slanina, Ph.D. (cvičící)
Mgr. Jindra Smutná, Ph.D. (cvičící)
prof. RNDr. Eva Táborská, CSc. (cvičící)
doc. RNDr. Josef Tomandl, Ph.D. (cvičící)
Mgr. Marie Tomandlová, Ph.D. (cvičící)
Martina Hanousková (pomocník)
Miriam Minaříková (pomocník)
Mgr. Lucie Novotná (pomocník)
Garance
prof. RNDr. Eva Táborská, CSc.
Biochemický ústav – Teoretická pracoviště – Lékařská fakulta
Kontaktní osoba: Monika Šudáková
Dodavatelské pracoviště: Biochemický ústav – Teoretická pracoviště – Lékařská fakulta
Rozvrh seminárních/paralelních skupin
aVLBC0422c/30: Út 8:00–10:30 A16/212, M. Hlaváčová, M. Tomandlová
aVLBC0422c/31: Čt 14:00–16:30 A16/216, H. Paulová, O. Peš
aVLBC0422c/32: Pá 8:00–10:30 A16/212, M. Králíková, J. Tomandl
aVLBC0422c/33: Čt 11:00–13:30 A16/216, J. Slanina, M. Tomandlová
aVLBC0422c/34: Čt 14:00–16:30 A16/212, O. Peš, J. Slanina
aVLBC0422c/35: Út 11:00–13:30 A16/216, O. Peš, J. Smutná
aVLBC0422c/36: Út 11:00–13:30 A16/212, J. Gregorová, O. Peš
aVLBC0422c/37: Út 8:00–10:30 A16/216, H. Paulová, M. Tomandlová
aVLBC0422c/38: Pá 8:00–10:30 A16/216, M. Králíková
aVLBC0422c/39: Čt 11:00–13:30 A16/212, H. Paulová, M. Tomandlová
Předpoklady
aVLBI0222p Biologie II - přednáška && aVLBF011p Biophysics - lecture && aVLBC0321s Biochemie I - sem. && aVLBC0321c Biochemie I - cvičení && aVLFY0321c Fyziologie I - cv. && aVLFY0321s Fyziologie I - sem.
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
Course is complementary to aVLBC0421p and aVLLC0421s. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics.
Výstupy z učení
The student is able:
- to explain the ways of obtaining biological material for biochemical analysis and is familiar with the rules of manipulation.
- to describe physiological values of the most important biochemical parameters.
- to interpret the results of biochemical analyzes, discusses the significance of the findings.
- to understand the principles of the most important biochemical-analytical methodologies.
- to be oriented in basic laboratory manipulations.
Osnova
  • • Directions for laboratory work. Handling biological material. Laboratory examination. Blood, plasma, serum. Blood collection. Blood processing. Manual methods, pipetting. Biochemical analyzers. Test requisition forms. • Investigations of glucose metabolism. Diabetes mellitus, Enzymatic determination of plasma glucose. Determination of glycaemia by personal glucometer. Oral glucose tolerance test (oGTT). Detection and determination of glucose in urine. Detection of ketone bodies in urine. Determination of glycated hemoglobin (HbA1c). • Investigation of lipid and cholesterol metabolism. Enzymatic determination of serum total cholesterol. Determination of blood cholesterol using Accutrend. Determination of blood triacylglycerols using Accutrend. Determination of HDL-cholesterol and LDL-cholesterol. Calculation of LDL-cholesterol and derived parameters. • Investigations in liver disease I – Enzymes. Determination of catalytic concentrations of aminotranferases in serum. Determination of ALP catalytic concentration in serum. • Selected investigations in liver disease II – Bile pigments and albumin. Determination of total bilirubin in serum. Detection of bilirubin in urine. Detection of urobilinogens in urine. Determination of serum albumin. • Investigation of plasma proteins. Determination of total protein by the biuret test. Determination of albumin in serum. Electrophoresis of serum proteins. Assay for increased level of C-reactive protein. Determination of serum IgE by ELISA method. • Biochemical tests of renal function – I. Investigation of urine by inspection and physical methods. Determination of creatinine in serum and urine. Estimation of glomerular filtration rate from creatinine clearance. Fractional reabsorption and excretion of water. • Biochemical tests of renal function – II. Detection of proteinuria. Urine test for haem compounds. Determination of protein concentration in urine. Detection of microalbuminuria. Detection of Bence Jones protein in urine. Determination of urea in serum and urine. Approximate assessment of the nitrogen balance. • Routine urinalysis. Test strips. Multipurpose diagnostic strips in urinalysis. Detection of addictive drugs in urine. Detection of barbiturates in urine. Detection of luteinizing hormone in urine. • Examinations in urolithiasis. Solubility of uric acid. Detection of uric acid by murexide reaction. Simplified chemical analysis of renal calculi. Determination of uric acid in serum and urine. • Some investigations of the gastrointestinal tract. Selected examinations of stomach functions. Laboratory tests in pancreas diseases. Test of occult bleeding in gastrointestinal tract.
Literatura
  • Texts available in Information System.
Výukové metody
laboratory practicals
Metody hodnocení
Full attendance in all practicals is the condition for obtaining of course-unit credits.
Vyučovací jazyk
Angličtina
Další komentáře
Předmět je vyučován každoročně.
Nachází se v prerekvizitách jiných předmětů
Předmět je zařazen také v obdobích jaro 2017, jaro 2018, jaro 2019, jaro 2021, jaro 2022, jaro 2023, jaro 2024, jaro 2025.