aVLBC0422c Biochemie II - cvičení

Lékařská fakulta
jaro 2021
Rozsah
0/3/0. 45. 2 kr. Ukončení: z.
Vyučující
doc. RNDr. Jiří Dostál, CSc. (cvičící)
Mgr. Jana Gregorová, Ph.D. (cvičící)
MUDr. Miroslava Hlaváčová, Ph.D. (cvičící)
MUDr. Michaela Králíková, Ph.D. (cvičící)
RNDr. Hana Paulová, CSc. (cvičící)
doc. Mgr. Ondřej Peš, Ph.D. (cvičící)
Mgr. Jiří Slanina, Ph.D. (cvičící)
Mgr. Jindra Smutná, Ph.D. (cvičící)
prof. RNDr. Eva Táborská, CSc. (cvičící)
doc. RNDr. Josef Tomandl, Ph.D. (cvičící)
Mgr. Marie Tomandlová, Ph.D. (cvičící)
Martina Hanousková (pomocník)
Miriam Minaříková (pomocník)
Garance
prof. RNDr. Eva Táborská, CSc.
Biochemický ústav – Teoretická pracoviště – Lékařská fakulta
Dodavatelské pracoviště: Biochemický ústav – Teoretická pracoviště – Lékařská fakulta
Rozvrh seminárních/paralelních skupin
aVLBC0422c/30: Čt 11:00–13:30 A16/212, H. Paulová
aVLBC0422c/31: Út 11:00–13:30 A16/211, M. Králíková, J. Smutná
aVLBC0422c/32: Pá 8:00–10:30 A16/212, J. Slanina, J. Tomandl
aVLBC0422c/33: Pá 8:00–10:30 A16/211, J. Gregorová, J. Tomandl
aVLBC0422c/34: Čt 11:00–13:30 A16/211, H. Paulová, M. Tomandlová
aVLBC0422c/35: Čt 14:00–16:30 A16/211, J. Slanina
aVLBC0422c/36: Čt 14:00–16:30 A16/212, H. Paulová
aVLBC0422c/37: Út 8:00–10:30 A16/212, J. Slanina, M. Tomandlová
aVLBC0422c/38: Út 11:00–13:30 A16/212, M. Králíková, O. Peš
aVLBC0422c/39: Út 8:00–10:30 A16/211, H. Paulová, J. Slanina
Předpoklady
aVLBI0222p Lékařská biologie II - př && aVLBF011p Biophysics - lecture && aVLBC0321s Biochemie I - sem. && aVLBC0321c Biochemie I - cvičení && aVLFY0321c Fyziologie I - cv. && aVLFY0321s Fyziologie I - sem.
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
Course is complementary to aVLBC0421p and aVLLC0421s. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics.
Výstupy z učení
The student is able:
- to explain the ways of obtaining biological material for biochemical analysis and is familiar with the rules of manipulation.
- to describe physiological values of the most important biochemical parameters.
- to interpret the results of biochemical analyzes, discusses the significance of the findings.
- to understand the principles of the most important biochemical-analytical methodologies.
- to be oriented in basic laboratory manipulations.
Osnova
  • • Directions for laboratory work. Handling biological material. Laboratory examination. Blood, plasma, serum. Blood collection. Blood processing. Manual methods, pipetting. Biochemical analyzers. Test requisition forms. • Investigations of glucose metabolism. Diabetes mellitus, Enzymatic determination of plasma glucose. Determination of glycaemia by personal glucometer. Oral glucose tolerance test (oGTT). Detection and determination of glucose in urine. Detection of ketone bodies in urine. Determination of glycated hemoglobin (HbA1c). • Investigation of lipid and cholesterol metabolism. Enzymatic determination of serum total cholesterol. Determination of blood cholesterol using Accutrend. Determination of blood triacylglycerols using Accutrend. Determination of HDL-cholesterol and LDL-cholesterol. Calculation of LDL-cholesterol and derived parameters. • Investigations in liver disease I – Enzymes. Determination of catalytic concentrations of aminotranferases in serum. Determination of ALP catalytic concentration in serum. • Selected investigations in liver disease II – Bile pigments and albumin. Determination of total bilirubin in serum. Detection of bilirubin in urine. Detection of urobilinogens in urine. Determination of serum albumin. • Investigation of plasma proteins. Determination of total protein by the biuret test. Determination of albumin in serum. Electrophoresis of serum proteins. Assay for increased level of C-reactive protein. Determination of serum IgE by ELISA method. • Biochemical tests of renal function – I. Investigation of urine by inspection and physical methods. Determination of creatinine in serum and urine. Estimation of glomerular filtration rate from creatinine clearance. Fractional reabsorption and excretion of water. • Biochemical tests of renal function – II. Detection of proteinuria. Urine test for haem compounds. Determination of protein concentration in urine. Detection of microalbuminuria. Detection of Bence Jones protein in urine. Determination of urea in serum and urine. Approximate assessment of the nitrogen balance. • Routine urinalysis. Test strips. Multipurpose diagnostic strips in urinalysis. Detection of addictive drugs in urine. Detection of barbiturates in urine. Detection of luteinizing hormone in urine. • Examinations in urolithiasis. Solubility of uric acid. Detection of uric acid by murexide reaction. Simplified chemical analysis of renal calculi. Determination of uric acid in serum and urine. • Some investigations of the gastrointestinal tract. Selected examinations of stomach functions. Laboratory tests in pancreas diseases. Test of occult bleeding in gastrointestinal tract.
Literatura
  • Texts available in Information System.
Výukové metody
laboratory practicals
Metody hodnocení
Conditions of giving the credits • Adequate preparation for the class, presented by completed pre-lab questions in protocols. • Submission of all completed protocols to the IS Homework Vaults in relevant dates. • Required attendance in classes. • All absences must be made up according to teacher instructions.
Vyučovací jazyk
Angličtina
Informace učitele
Practical lessons (practicals) will take place mainly in contact form in odd teaching weeks, always in the time according to the schedule and syllabus. Materials for practicals will always be included in Study materials of the course. For each practical, there are instructions for performing practical tasks with relevant theoretical background and a template (protocol), in which students will write the measured results and conclusions. Before each class, students must study the relevant instructions for the procedures and complete answers to the pre-lab questions in the template (protocol). Students will bring printed instructions and protocols with answers or adequate materials on tablets. The results of practical tasks, calculations, and answers to questions are recorded by students directly during the class into protocols. Further practical information is given in the file "Directions for laboratory work". After the class, students will definitely complete the protocol and upload it to IS Homework Vault before the specified deadline. Classes in the 8th and 13th week will take place online via the MS-TEAMS application. During these meetings, there will be a discussion of the theoretical background and conclusions from the exercises, the elaboration of protocols and a discussion of questions to students - always on the content of previous exercises. Attendance in all practical classes (contact and online) is mandatory, absences must be excused through the study department within 5 days of the beginning of absence. Missed classes must be substituted in agreement with the teacher. A white coat, slippers or sturdy shoes with overshoes, a FFP2 respirator, gloves, and a calculator are required for the practical class.
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