aVLBC0422c Biochemie II - cvičení

Lékařská fakulta
jaro 2022
Rozsah
0/3/0. 45. 3 kr. Ukončení: z.
Vyučující
Mgr. Jana Gregorová, Ph.D. (cvičící)
MUDr. Michaela Králíková, Ph.D. (cvičící)
RNDr. Hana Paulová, CSc. (cvičící)
doc. Mgr. Ondřej Peš, Ph.D. (cvičící)
Mgr. Jiří Slanina, Ph.D. (cvičící)
Mgr. Jindra Smutná, Ph.D. (cvičící)
doc. RNDr. Josef Tomandl, Ph.D. (cvičící)
Mgr. Marie Tomandlová, Ph.D. (cvičící)
doc. RNDr. Jiří Dostál, CSc. (pomocník)
Mgr. David Galuška (pomocník)
Martina Hanousková (pomocník)
doc. PharmDr. Jiří Kos, Ph.D. (pomocník)
Miriam Minaříková (pomocník)
Bc. Ivana Ševčíková (pomocník)
prof. RNDr. Eva Táborská, CSc. (pomocník)
Garance
doc. RNDr. Josef Tomandl, Ph.D.
Biochemický ústav – Teoretická pracoviště – Lékařská fakulta
Kontaktní osoba: RNDr. Hana Paulová, CSc.
Dodavatelské pracoviště: Biochemický ústav – Teoretická pracoviště – Lékařská fakulta
Rozvrh seminárních/paralelních skupin
aVLBC0422c/28: Čt 11:00–13:30 A16/212, J. Slanina
aVLBC0422c/29: Čt 11:00–13:30 A16/216, H. Paulová
aVLBC0422c/30: Čt 11:00–13:30 A16/211, M. Králíková
aVLBC0422c/31: Út 11:00–13:30 A16/211, O. Peš
aVLBC0422c/32: Út 8:00–10:30 A16/211, M. Tomandlová
aVLBC0422c/33: Út 8:00–10:30 A16/212, H. Paulová
aVLBC0422c/34: St 11:00–13:30 A16/211, J. Smutná
aVLBC0422c/35: St 11:00–13:30 A16/216, J. Gregorová
aVLBC0422c/36: Út 11:00–13:30 A16/216, H. Paulová
aVLBC0422c/37: St 11:00–13:30 A16/212, O. Peš
aVLBC0422c/38: Út 11:00–13:30 A16/212, J. Slanina
aVLBC0422c/39: Út 8:00–10:30 A16/216, J. Slanina
Předpoklady
aVLBC0321s Biochemistry I - seminar && aVLFY0321c Fyziologie I - cv. && aVLFY0321s Fyziologie I - sem.
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
Course is complementary to aVLBC0422p. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics.
Výstupy z učení
The student is able:
- to explain the ways of obtaining biological material for biochemical analysis and is familiar with the rules of manipulation.
- to describe physiological values of the most important biochemical parameters.
- to interpret the results of biochemical analyzes, discusses the significance of the findings.
- to understand the principles of the most important biochemical-analytical methodologies.
- to be oriented in basic laboratory manipulations.
Osnova
  • Organization of work in practical exercises. Handling with biological material.
  • Blood collection and processing.
  • Pipetting.
  • Communication of biochemical test results.
  • Determination of plasma glucose, determination of glycemia by personal glucometer. Oral glucose tolerance test (oGTT). Evidence of glucosuria and ketonuria. Determination of glycated hemoglobin.
  • Determination of total cholesterol in serum and blood. Determination of triacylglycerols in blood. Determination of HDL- and LDL-cholesterol. Calculation of LDL-cholesterol and derived parameters. Determination of catalytic concentrations of aminotransferases in serum.
  • Determination of total serum bilirubin. Detection of bilirubin and urobilinogens in urine.
  • Determination of serum albumin.
  • Determination of total protein and serum albumin. Serum protein electrophoresis.
  • Detection and determination of C-reactive protein in serum and blood. Determination of ferritin in serum.
  • Objective and physical examination of urine.
  • Determination of creatinine in serum and urine. Determination of glomerular filtration from creatinine clearance. Calculation of fractional water resorption and excretion.
  • Proof and determination of proteinuria and albuminuria. Evidence of blood pigment in the urine. Detection of Bence-Jones protein in urine.
  • Determination of urea in serum and urine. Nitrogen balance.
  • Examination of urine with multifunctional diagnostic strips. Demonstration of drugs in urine. Detection of luteinizing hormone in urine.
  • Solubility of uric acid. Orientational chemical analysis of urinary stones.
  • Determination of uric acid in serum and urine.
  • Selected laboratory examinations of the stomach. Laboratory tests for pancreatic diseases Digestive tract occult bleeding test.
Literatura
    povinná literatura
  • Instructions available in IS MUNI.
Výukové metody
Laboratory practical classes
Metody hodnocení
Course-unit credit.
Conditions for giving the course-unit credit (all conditions should be met):
  • completion of all classes,
  • elaboration of all protocols and insertion into the Homework vaults in IS MUNI,
  • successful completion of the credit test (at least 70% of points),
  • course-unit credit from aZC041.
    The detailed conditions are posted in IS MUNI (Study materials – Course-related instructions).
    • Vyučovací jazyk
    Angličtina
    Informace učitele
    https://is.muni.cz/auth/el/1411/jaro2022/aVLBC0422c/
    Information to practical exercises, the conditions for giving the course-unit credit and the syllabus are available in the IS MUNI in the Course-related instructions. The syllabus is also posted on the notice board of the Department of Biochemistry.
    A white coat, slippers or sturdy shoes with overshoes, a respirator type FFP2, gloves, a calculator are required for the exercise.
    Consultations are possible after a personal arrangement with the teacher.
    Další komentáře
    Předmět je vyučován každoročně.
    Successful completion of the course is a condition for access to the exam in the course aVLBC0422p.
    Nachází se v prerekvizitách jiných předmětů
    Předmět je zařazen také v obdobích jaro 2017, jaro 2018, jaro 2019, jaro 2020, jaro 2021, jaro 2023, jaro 2024, jaro 2025.