aVLBC0321s Biochemie I - seminář

Lékařská fakulta
podzim 2019
Rozsah
0/2/0. 30. 4 kr. Ukončení: z.
Vyučující
doc. RNDr. Jiří Dostál, CSc. (cvičící)
MUDr. Michaela Králíková, Ph.D. (cvičící)
RNDr. Hana Paulová, CSc. (cvičící)
Mgr. Jiří Slanina, Ph.D. (cvičící)
prof. RNDr. Eva Táborská, CSc. (cvičící)
doc. RNDr. Josef Tomandl, Ph.D. (cvičící)
Mgr. Marie Tomandlová, Ph.D. (cvičící)
MUDr. Miroslava Hlaváčová, Ph.D. (pomocník)
PharmDr. Katarína Kostolanská (pomocník)
Lenka Nerudová (pomocník)
Mgr. Roman Sándor, Ph.D. (pomocník)
Garance
prof. RNDr. Eva Táborská, CSc.
Biochemický ústav – Teoretická pracoviště – Lékařská fakulta
Dodavatelské pracoviště: Biochemický ústav – Teoretická pracoviště – Lékařská fakulta
Rozvrh seminárních/paralelních skupin
aVLBC0321s/30: Út 16:00–17:40 A16/213, J. Tomandl
aVLBC0321s/31: Út 16:00–17:40 A16/213, J. Tomandl
aVLBC0321s/32: Út 14:00–15:40 A16/215, J. Dostál
aVLBC0321s/33: Út 14:00–15:40 A16/215, J. Dostál
aVLBC0321s/34: Po 16:00–17:40 A16/215, H. Paulová
aVLBC0321s/35: Po 16:00–17:40 A16/215, H. Paulová
aVLBC0321s/36: Út 12:00–13:40 A16/215
aVLBC0321s/37: Út 12:00–13:40 A16/215
aVLBC0321s/38: Po 13:00–14:40 A16/215, J. Slanina
aVLBC0321s/39: Po 13:00–14:40 A16/215, J. Slanina
Předpoklady
aVLBI0222c Biologie II - cvičení && aVLBF011c Biophysics - practice
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
Seminars (A) and practicals (B) have a common content. The aim of the course is to obtain knowledge on essential metabolic processes on the cellular level. Understanding of these proceses is a base for comprihension of metabolism on the tissue and organ level. Introductory lessons summarize the basic terms of chemistry needed for understanding of body structure a physico-chemical processes occuring in it (chemical composition of the body, biologically important elements, water, electrolytes, non-electrolytes, osmotic pressure, acid-base, redox and precipitation reactions), the following lectures are focused on biochemichal pathways in cells.
Výstupy z učení
At the end of the course students will be competent to:
- understand the meaning of basic chemical terms (pH, osmolality, electrolyte, buffer, etc.) and apply this knowledge when describing the properties of body fluids.
- describe the role of macro/microbiogenic elements in the body.
- discusse the properties and function of enzymes
- describe basic catabolic and anabolic pathways of carbohydrates, lipids, and protein metabolism, and their relationships.
- understand the principles of energy production, utilization and deposition at the cellular level.
- explain the function of cell membranes and the principle of compartmentalization at the cellular level and the transport processes on the membrane.
- describe protein synthesis, starting with the replication and transcription, translation and post-translational modifications. Understand the relationship between protein structure and function.
- explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
- discusse the principles of some diseases at the molecular level.
Osnova
  • 1A: Introduction to biochemistry.
  • 1B: Electrolytes, osmolality, tonicity, oncotic pressure, osmolal gap.
  • 2A: Acid-base reactions, pH of body fluids.
  • 2B: Buffers, Henderson-Hasselbalch equation.
  • 3A: TEST 1 (sem 1A-2B). Biochemically important organic compounds I (alcohols, aldehydes).
  • 3B: Biochemically important organic compounds II (carboxylic acids and derivatives).
  • 4A: Bioenergetics, Gibbs energy, ATP, redox reactions.
  • 4B: Enzymes, kinetics, saturation curve, inhibition.
  • 5A: Cofactors of enzymes, relation to vitamins.
  • 5B: Citrate cycle, respiratory chain.
  • 6A: Saccharides, structures, nutrition, digestion. Transport of glucose into cells. Glycolysis.
  • 6B: Metabolism of glycogen. Metabolism of fructose and galactose.
  • 7A: TEST 2 (sem 3A-6B). Surfactants (types, physiological roles). Lipids (structures).
  • 7B: Cell membranes, transport.
  • 8A: Lipids in nutrition, digestion. Catabolism of fatty acids, ketone bodies.
  • 8B: Desaturation of fatty acids. Cholesterol.
  • 9A: Metabolism of bile acids, eicosanoids, phospholipids.
  • 9B: Lipophilic vitamins, ROS. Lipoperoxidation.
  • 10A: TEST 3 (sem 7A-9B). Amino acids, proteins, structure, properties.
  • 10B: Hemoglobin, types, function, abnormal types.
  • 11A: Digestion of proteins, general features of amino acid metabolism, synthesis of urea.
  • 11B: Metabolism of amino acids I (catabolism, synthesis of non-essential AA, congenital disorders).
  • 12A: Metabolism of amino acids II (conversions to special products, heme).
  • 12B: TEST 4 (sem 10A-12A). Structure of bases, nucleosides, nucleotides, roles of nucleotides. Metabolism of purine and pyrimidine bases I.
  • 13A: Metabolism of purine and pyrimidine bases II.
  • 13B: DNA, RNA structure, replication, transcription.
  • 14A: Protein synthesis. Post-translation modification, collagen synthesis. Credit test.
  • 14B: Consultations, compensatory lessons.
Literatura
    povinná literatura
  • Lecture files including lecture notes available in IS
  • Seminar texts available in Information system
  • RODWELL, Victor W., David A. BENDER, Kathleen M. BOTHAM, Peter J. KENNELLY a P. Anthony WEIL. Harper's illustrated biochemistry. Thirty-first edition. New York: McGraw-Hill, 2018, x, 789. ISBN 9781260288421. info
  • KOOLMAN, Jan a Klaus-Heinrich ROEHM. Color Atlas of Biochemistry. 3rd ed. Georg Thieme Verlag, 2013. ISBN 978-3-13-100373-7. info
Výukové metody
Course is based on group discusion to the given topics. The outlines of discusion are in the recommended textbook. Complementary materials are available in section Study materials.
Metody hodnocení
Full attendance in seminars is the principal condition. If any absence, it must be apologized through Department of Study Affairs up to five days. If apology is recorded in Information System, then student is allowed to make up the absence according to teacher's instructions. Four revision tests are written in seminars, semestral limit for credit is 42. If the semestral limit is not fulfilled, student must write the Credit test (limit 14/30). All absences must be made up before writing the credit test. One repetition of the Credit test is approved.
Vyučovací jazyk
Angličtina
Další komentáře
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Předmět je zařazen také v obdobích podzim 2016, podzim 2017, podzim 2018, podzim 2020, podzim 2021, podzim 2022, podzim 2023, podzim 2024.