BLLC0111c Medical Chemistry - practice

Faculty of Medicine
autumn 2023
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. David Galuška (seminar tutor)
Mgr. Jan Víteček, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
doc. Mgr. Zdenka Fohlerová, Ph.D. (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
Martina Hanousková (assistant)
doc. PharmDr. Jiří Kos, Ph.D. (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Mgr. Jindra Smutná, Ph.D. (assistant)
PharmDr. Kristýna Šebrlová, Ph.D. (assistant)
prof. RNDr. Eva Táborská, CSc. (assistant)
doc. RNDr. Josef Tomandl, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
BLLC0111c/Sk_1: Thu 21. 9. 13:00–15:30 A16/216, Thu 5. 10. 13:00–15:30 A16/216, Thu 12. 10. 13:00–15:30 A16/216, Thu 19. 10. 13:00–15:30 A16/216, Thu 26. 10. 13:00–15:30 A16/216, Thu 2. 11. 13:00–15:30 A16/216, Thu 9. 11. 13:00–15:30 A16/216, Thu 16. 11. 13:00–15:30 A16/216, Thu 23. 11. 13:00–15:30 A16/216, Thu 30. 11. 13:00–15:30 A16/216, Thu 7. 12. 13:00–15:30 A16/216, Thu 14. 12. 13:00–15:30 A16/216, Thu 21. 12. 13:00–15:30 A16/216
BLLC0111c/Sk_2: Thu 21. 9. 13:00–15:30 A16/212, Thu 5. 10. 13:00–15:30 A16/212, Thu 12. 10. 13:00–15:30 A16/212, Thu 19. 10. 13:00–15:30 A16/212, Thu 26. 10. 13:00–15:30 A16/212, Thu 2. 11. 13:00–15:30 A16/212, Thu 9. 11. 13:00–15:30 A16/212, Thu 16. 11. 13:00–15:30 A16/212, Thu 23. 11. 13:00–15:30 A16/212, Thu 30. 11. 13:00–15:30 A16/212, Thu 7. 12. 13:00–15:30 A16/212, Thu 14. 12. 13:00–15:30 A16/212, Thu 21. 12. 13:00–15:30 A16/212
Prerequisites
Participation in the course is conditional on the successful completion of the course ZC011.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main objectives of the course are to become oriented in the environment of the chemical laboratory and its equipment, to acquire simple laboratory procedures and techniques, to practice common separation procedures and become familiar with handling standard laboratory machines, to teach students how to process measurement results into protocols, evaluate them and draw conclusions.
Learning outcomes
Students
- will be familiar with regulations for safe handling, use, and disposal of chemicals
- will be able to handle with basal laboratory equipment and follow proper procedures
- will pipette and weigh according to the principles of good laboratory practice
- will understand the relationship between the composition of different solutions and their pH value
- will be able to describe the general composition and role of the buffers, and prepare a buffer
- to describe the essence of the formation of insoluble and poorly soluble compounds, to identify factors that can influence the insolubility of compounds
- will be oriented in relations between a structure od bioorganic molecules and their reactivity
- will explain principles of chromatographic methods, their applications and utilization
- will be able to apply photometric methods for determination of solution concentration
Syllabus
  • Introduction, organizational instructions. Safety and health protection when working in the laboratory, principles of first aid.
  • Measuring containers/tools, plastic. Heating, cooling, weighing. Pressure cylinders.
  • Measuring small volumes of liquids, calibration of pipettors, expression of accuracy and correctness.
  • Preparation of solutions with a given concentration.
  • Filtration, dialysis, ultrafiltration, centrifugation. Concentration of samples.
  • Measurement and calculation of pH solutions of acids, bases and salts, hydrolysis, pH-meters.
  • Neutralization quantitative analysis. Titration neutralization curves.
  • Buffer solutions and methods of their preparation. Determination of buffering capacity.
  • Ion reactions, ions in drinking water, ion-selective electrodes, solubility of substances.
  • Chromatography – basic types and techniques. Demineralization of water.
  • Spectrophotometry.
  • Reactions of organic compounds (oxidation of alcohols, proof of aldehydes, colour reactions of amino acids).
  • Solubility of proteins, methods of their quantification.
Literature
    required literature
  • Interaktivní osnova v IS MUNI.
Teaching methods
The course is realized in laboratories. Students are doing simple experiments according to the instructions in the textbook. On the base of results, they prepare the lab-reports.
Assessment methods
Course-unit credit. Conditions for giving course-unit credit :
  • Completion of all exercises, incl. submission of all lab reports.
  • Completion of progress tests.
    Failure to meet the requirements of the progress tests requires a remedial test which may be repeated once.
    Detailed conditions are published in IS MUNI (Study materials – Course-related instructions).
    • Language of instruction
    Czech
    Further comments (probably available only in Czech)
    The course is taught annually.
    Information on the extent and intensity of the course: 45.
    Listed among pre-requisites of other courses
    Teacher's information
    A white coat, slippers or sturdy shoes with overshoes, gloves, and a calculator are required for the practical class.
    The course is also listed under the following terms Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2024.
    • Enrolment Statistics (autumn 2023, recent)
    • Permalink: https://is.muni.cz/course/med/autumn2023/BLLC0111c