Course Information

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).
In-person direct teaching

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 57/70, only registered with preference (fields directly associated with the programme): 27/70

fields of study / plans the course is directly associated with

there are 11 fields of study the course is directly associated with, display

Course objectives

The main purpose of the course is to provide students with a general overview of the basic techniques of electron microscopy and of the possibilities of processing biological material for electron microscopes.

Learning outcomes

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Mon 19. 2. to Sun 26. 5. Wed 14:00–15:50 B11/306

• Timetable of Seminar Groups:

Bi3010/01: Mon 19. 2. to Sun 26. 5. Tue 9:00–10:50 D31/1S11, I. Hodová
Bi3010/02: Mon 19. 2. to Sun 26. 5. Tue 11:00–12:50 D31/1S11, I. Hodová
Bi3010/03: Mon 19. 2. to Sun 26. 5. Tue 14:00–15:50 D31/1S11, I. Hodová
Bi3010/04: Mon 19. 2. to Sun 26. 5. Wed 9:00–10:50 D31/1S11, I. Hodová
Bi3010/05: Mon 19. 2. to Sun 26. 5. Wed 11:00–12:50 D31/1S11, I. Hodová
Bi3010/06: Mon 19. 2. to Sun 26. 5. Wed 14:00–15:50 D31/1S11, I. Hodová

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 35/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 10 fields of study the course is directly associated with, display

Course objectives

The main purpose of the course is to provide students with a general overview of the basic techniques of electron microscopy and of the possibilities of processing biological material for electron microscopes.

Learning outcomes

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Mon 12:00–13:50 B11/306

• Timetable of Seminar Groups:

Bi3010/01: Tue 9:00–10:50 D31/1S11, I. Hodová
Bi3010/02: Tue 11:00–12:50 D31/1S11, I. Hodová
Bi3010/03: Tue 14:00–15:50 D31/1S11, I. Hodová
Bi3010/04: Wed 9:00–10:50 D31/1S11, I. Hodová
Bi3010/05: Wed 11:00–12:50 D31/1S11, I. Hodová
Bi3010/06: Wed 14:00–15:50 D31/1S11, I. Hodová

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 12/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 11 fields of study the course is directly associated with, display

Course objectives

The main purpose of the course is to provide students with a general overview of the basic techniques of electron microscopy and of the possibilities of processing biological material for electron microscopes.

Learning outcomes

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Mon 12:00–13:50 B11/306

• Timetable of Seminar Groups:

Bi3010/01: Tue 9:00–10:50 D31/1S11, I. Hodová
Bi3010/02: Tue 11:00–12:50 D31/1S11, I. Hodová
Bi3010/03: Tue 14:00–15:50 D31/1S11, I. Hodová
Bi3010/04: Wed 9:00–10:50 D31/1S11, I. Hodová
Bi3010/05: Wed 11:00–12:50 D31/1S11, I. Hodová
Bi3010/06: Wed 14:00–15:50 D31/1S11, I. Hodová

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 4/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 10 fields of study the course is directly associated with, display

Course objectives

The main purpose of the course is to provide students with a general overview of the basic techniques of electron microscopy and of the possibilities of processing biological material for electron microscopes.

Learning outcomes

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Mon 1. 3. to Fri 14. 5. Mon 12:00–13:50 online_B4

• Timetable of Seminar Groups:

Bi3010/B1: Tue 1. 6. 8:00–12:00 Kontaktujte učitele, 13:00–17:00 Kontaktujte učitele, Mon 21. 6. 8:00–12:00 Kontaktujte učitele
Bi3010/B2: Wed 2. 6. 8:00–12:00 Kontaktujte učitele, 13:00–17:00 Kontaktujte učitele, Mon 21. 6. 13:00–17:00 Kontaktujte učitele
Bi3010/B3: Thu 3. 6. 8:00–12:00 Kontaktujte učitele, 13:00–17:00 Kontaktujte učitele, Tue 22. 6. 8:00–12:00 Kontaktujte učitele
Bi3010/B4: Tue 8. 6. 8:00–12:00 Kontaktujte učitele, 13:00–17:00 Kontaktujte učitele, Tue 22. 6. 13:00–17:00 Kontaktujte učitele
Bi3010/B5: Wed 9. 6. 8:00–12:00 Kontaktujte učitele, 13:00–17:00 Kontaktujte učitele, Wed 23. 6. 8:00–12:00 Kontaktujte učitele
Bi3010/B6: Thu 10. 6. 8:00–12:00 Kontaktujte učitele, 13:00–17:00 Kontaktujte učitele, Wed 23. 6. 13:00–17:00 Kontaktujte učitele

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 10 fields of study the course is directly associated with, display

Course objectives

The main purpose of the course is to provide students with a general overview of the basic techniques of electron microscopy and of the possibilities of processing biological material for electron microscopes.

Learning outcomes

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Mon 17:00–18:50 B11/306

• Timetable of Seminar Groups:

Bi3010/01: Thu 11:00–12:50 D31/1S11, I. Hodová
Bi3010/02: Thu 14:00–15:50 D31/1S11, I. Hodová
Bi3010/03: Thu 9:00–10:50 D31/1S11, I. Hodová
Bi3010/04: Tue 9:00–10:50 D31/1S11, I. Hodová
Bi3010/05: Tue 14:00–15:50 D31/1S11, I. Hodová
Bi3010/06: Tue 11:00–12:50 D31/1S11, I. Hodová

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 10 fields of study the course is directly associated with, display

Course objectives

The main purpose of the course is to provide students with a general overview of the basic techniques of electron microscopy and of the possibilities of processing biological material for electron microscopes.

Learning outcomes

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Mon 18. 2. to Fri 17. 5. Mon 17:00–18:50 B11/306

• Timetable of Seminar Groups:

Bi3010/01: Mon 18. 2. to Fri 17. 5. Tue 9:00–10:50 D31/1S11, I. Hodová
Bi3010/02: Mon 18. 2. to Fri 17. 5. Tue 11:00–12:50 D31/1S11, I. Hodová
Bi3010/03: Mon 18. 2. to Fri 17. 5. Tue 14:00–15:50 D31/1S11, I. Hodová
Bi3010/04: Mon 18. 2. to Fri 17. 5. Thu 9:00–10:50 D31/1S11, I. Hodová
Bi3010/05: Mon 18. 2. to Fri 17. 5. Thu 11:00–12:50 D31/1S11, I. Hodová
Bi3010/06: Mon 18. 2. to Fri 17. 5. Thu 14:00–15:50 D31/1S11, I. Hodová

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Anim. phylog. & divers. || Bi2030 High. plant phylog. & divers. || Bi2090 Verteb. phylog. & divers. || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

The main purpose of the course is to provide students with a general overview of the basic techniques of electron microscopy and of the possibilities of processing biological material for electron microscopes.

Learning outcomes

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)
Mgr. Naděžda Vaškovicová, Ph.D. (seminar tutor)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Mon 17:00–18:50 B11/306

• Timetable of Seminar Groups:

Bi3010/01: Thu 9:00–10:50 D31/1S11, I. Hodová, N. Vaškovicová
Bi3010/02: Thu 14:00–15:50 D31/1S11, I. Hodová, N. Vaškovicová
Bi3010/03: Thu 11:00–12:50 D31/1S11, I. Hodová, N. Vaškovicová
Bi3010/04: Tue 11:00–12:50 D31/1S11, I. Hodová, N. Vaškovicová
Bi3010/05: Tue 14:00–15:50 D31/1S11, I. Hodová, N. Vaškovicová
Bi3010/06: Tue 9:00–10:50 D31/1S11, I. Hodová, N. Vaškovicová

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Anim. phylog. & divers. || Bi2030 High. plant phylog. & divers. || Bi2090 Verteb. phylog. & divers. || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

The main purpose of the course is to provide students with a general overview of the basic techniques of electron microscopy and of the possibilities of processing biological material for electron microscopes.

Learning outcomes

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)
Mgr. Naděžda Vaškovicová, Ph.D. (seminar tutor)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Mon 20. 2. to Mon 22. 5. Fri 8:00–9:50 B11/306

• Timetable of Seminar Groups:

Bi3010/01: Mon 20. 2. to Mon 22. 5. Tue 9:00–10:50 D31/1S11, I. Hodová
Bi3010/02: Mon 20. 2. to Mon 22. 5. Tue 11:00–12:50 D31/1S11, I. Hodová
Bi3010/03: Mon 20. 2. to Mon 22. 5. Tue 13:00–14:50 D31/1S11, I. Hodová
Bi3010/04: Mon 20. 2. to Mon 22. 5. Thu 9:00–10:50 D31/1S11, I. Hodová
Bi3010/05: Mon 20. 2. to Mon 22. 5. Thu 11:00–12:50 D31/1S11, I. Hodová
Bi3010/06: Mon 20. 2. to Mon 22. 5. Thu 13:00–14:50 D31/1S11, I. Hodová

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Anim. phylog. & divers. || Bi2030 High. plant phylog. & divers. || Bi2090 Verteb. phylog. & divers. || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 1/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)
Mgr. Naděžda Vaškovicová, Ph.D. (seminar tutor)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Tue 8:00–9:50 B11/306

• Timetable of Seminar Groups:

Bi3010/01: Tue 9:00–10:50 D31/1S11, I. Hodová
Bi3010/02: Tue 11:00–12:50 D31/1S11, I. Hodová
Bi3010/03: Tue 14:00–15:50 D31/1S11, I. Hodová
Bi3010/04: Wed 9:00–10:50 D31/1S11, I. Hodová
Bi3010/05: Wed 11:00–12:50 D31/1S11, I. Hodová

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Anim. phylog. & divers. || Bi2030 High. plant phylog. & divers. || Bi2090 Verteb. phylog. & divers. || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)
Mgr. Naděžda Vaškovicová, Ph.D. (seminar tutor)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Mon 13:00–14:50 B11/306

• Timetable of Seminar Groups:

Bi3010/01: Tue 9:00–10:50 D31/1S11, I. Hodová
Bi3010/02: Tue 11:00–12:50 D31/1S11, I. Hodová
Bi3010/03: Tue 14:00–15:50 D31/1S11, I. Hodová
Bi3010/04: Wed 9:00–10:50 D31/1S11, I. Hodová
Bi3010/05: Wed 11:00–12:50 D31/1S11, I. Hodová
Bi3010/06: Wed 14:00–15:50 D31/1S11, I. Hodová

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Anim. phylog. & divers. || Bi2030 High. plant phylog. & divers. || Bi2090 Verteb. phylog. & divers. || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: k (colloquium).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Mon 9:00–10:50 B11/306

• Timetable of Seminar Groups:

Bi3010/01: Wed 9:00–10:50 D31/1S11, I. Hodová
Bi3010/02: Wed 11:00–12:50 D31/1S11, I. Hodová
Bi3010/04: Thu 9:00–10:50 D31/1S11, I. Hodová
Bi3010/05: Thu 11:00–12:50 D31/1S11, I. Hodová
Bi3010/06: Thu 13:00–14:50 D31/1S11, I. Hodová

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Anim. phylog. & divers. || Bi2030 High. plant phylog. & divers. || Bi2090 Verteb. phylog. & divers. || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 7 fields of study the course is directly associated with, display

Course objectives

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electron microscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Mon 9:00–10:50 B11/306

• Timetable of Seminar Groups:

Bi3010/01: Wed 9:00–10:50 PARA, I. Hodová
Bi3010/02: Wed 11:00–12:50 PARA, I. Hodová
Bi3010/03: Wed 13:00–14:50 PARA, I. Hodová
Bi3010/04: Thu 9:00–10:50 PARA, I. Hodová
Bi3010/05: Thu 11:00–12:50 PARA, I. Hodová
Bi3010/06: Thu 13:00–14:50 PARA, I. Hodová

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Anim. phylog. & divers. || Bi2030 High. plant phylog. & divers. || Bi2090 Verteb. phylog. & divers. || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 13 fields of study the course is directly associated with, display

Course objectives

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe to the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain of the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledges to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electronmicroscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

colloquium

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable

Wed 8:00–9:50 BR3

• Timetable of Seminar Groups:

Bi3010/01: Wed 9:00–10:50 PARA, I. Hodová
Bi3010/02: Wed 11:00–12:50 PARA, I. Hodová
Bi3010/03: Wed 13:00–14:50 PARA, I. Hodová
Bi3010/04: Thu 9:00–10:50 PARA, I. Hodová
Bi3010/05: Thu 11:00–12:50 PARA, I. Hodová
Bi3010/06: Thu 13:00–14:50 PARA, I. Hodová

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Animal System and Evolution || Bi2030 System evol. higher plants || Bi2090 System evolution vertebrates || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 13 fields of study the course is directly associated with, display

Course objectives

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe to the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain of the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledges to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electronmicroscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

colloquium

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.

Timetable

Wed 16:00–17:50 BR3

• Timetable of Seminar Groups:

Bi3010/01: Wed 9:00–10:50 PARA, I. Hodová
Bi3010/02: Wed 11:00–12:50 PARA, I. Hodová
Bi3010/03: Wed 13:00–14:50 PARA, I. Hodová
Bi3010/04: Thu 9:00–10:50 PARA, I. Hodová
Bi3010/05: Thu 11:00–12:50 PARA, I. Hodová
Bi3010/06: Thu 13:00–14:50 PARA, I. Hodová

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Animal System and Evolution || Bi2030 System evol. higher plants || Bi2090 System evolution vertebrates || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 72 student(s).
Current registration and enrolment status: enrolled: 0/72, only registered: 0/72, only registered with preference (fields directly associated with the programme): 0/72

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe to the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain of the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledges to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electronmicroscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

colloquium

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 12 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.

Timetable

Fri 8:00–9:50 BR3

• Timetable of Seminar Groups:

Bi3010/01: Thu 13:00–14:50 PARA, I. Hodová
Bi3010/02: Thu 11:00–12:50 PARA, I. Hodová
Bi3010/03: Wed 12:00–13:50 PARA, I. Hodová
Bi3010/04: Wed 14:00–15:50 PARA, I. Hodová
Bi3010/05: Wed 10:00–11:50 PARA, I. Hodová
Bi3010/06: Thu 9:00–10:50 PARA, I. Hodová

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Animal System and Evolution || Bi2030 System evol. higher plants || Bi2090 System evolution vertebrates || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe to the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain of the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledges to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electronmicroscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

colloquium

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.

Timetable

Tue 17:00–18:50 BR3

• Timetable of Seminar Groups:

Bi3010/01: Mon 11:00–12:50 PARA, I. Hodová
Bi3010/02: Mon 13:00–14:50 PARA, I. Hodová
Bi3010/03: Tue 9:00–10:50 PARA, I. Hodová
Bi3010/04: Mon 9:00–10:50 PARA, I. Hodová
Bi3010/05: Tue 11:00–12:50 PARA, I. Hodová
Bi3010/06: Tue 13:00–14:50 PARA, I. Hodová

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Animal System and Evolution || Bi2030 System evol. higher plants || Bi2090 System evolution vertebrates || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 75 student(s).
Current registration and enrolment status: enrolled: 0/75, only registered: 0/75, only registered with preference (fields directly associated with the programme): 0/75

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

Main objectives can be summarized as follows: to introduce to the principles and application of the electron microscopy; to lern the history of the development of electron microscopes; to describe to the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain of the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledges to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electronmicroscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Assessment methods

colloquium

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.

Timetable

Fri 9:00–10:50 BR3

• Timetable of Seminar Groups:

Bi3010/01: Tue 9:00–10:50 Kontaktujte učitele
Bi3010/02: Tue 11:00–12:50 Kontaktujte učitele
Bi3010/03: Tue 13:00–14:50 Kontaktujte učitele
Bi3010/04: Wed 9:00–10:50 Kontaktujte učitele
Bi3010/05: Wed 11:00–12:50 Kontaktujte učitele
Bi3010/06: Wed 13:00–14:50 Kontaktujte učitele

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Animal System and Evolution || Bi2030 System evol. higher plants || Bi2090 System evolution vertebrates || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 80 student(s).
Current registration and enrolment status: enrolled: 0/80, only registered: 0/80, only registered with preference (fields directly associated with the programme): 0/80

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives (in Czech)

Předmětu je koncipován jako základní kurs seznamující posluchače s historií vývoje, základními konstrukčními principy a mechanismy tvorby obrazu v prozařovacím a rastrovacím elektronovém mikroskopu. Dále je pozornost věnována principům a nejdůležitějším zásadám přípravy preparátu pro oba typy mikroskopu.

Syllabus (in Czech)

• Teoretická část: Historie oboru, základní pojmy (urychlené el., rozlišovací schopnost, interakce el. s preparátem, zobrazovací systémy v el. mikroskopii, elektromagnetické čočky, vady čoček a jejich korekce) Transmisní el. mikroskop: konstrukce a princip tvorby obzazu (pružný a nepružný rozptyl, interference, kontrast Příprava preparátů pro TEM: fixace, odvodŇování, zalévání, řezání na ultramikrotomu, další metody: mrazové lámání a mrazové leptání, repliky. Rastrovací el. mikroskop: konstrukce zařízení, princip tvorby obrazu, zvětšení, rozlišení, hloubka ostrosti Příprava materiálu pro SEM: vlastnosti preparátu, sběr, fixace, odvodňování, sušení (metoda kritického bodu), pokovování (metoda iontového naprašování a vakuového napařování) Fotografická technika v el. mikroskopii Praktická část: Příprava skleněných nožů - lámání nožů na Knife-makeru, hodnocení kvality ostří, výroba vaničky na skleněný nůž Příprava formvarové fólie - potahování měděných sítěk a jejich sběr s vodní hladiny Práce na ultramikrotomu - samostatná příprava polotenkých řezů, jejich sběr na sklíčka, barvení a hodnocení. Demonstrace a samostatná obsluha TEM Demonstrace a samostatná obsluha SEM

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• DRAHOŠ, Vladimír and Armin DELONG. Praktická elektronová mikroskopie. 1. vyd. Praha: Nakladatelství Československé akademie věd, 1958, 370 s. info

Assessment methods (in Czech)

Výuka je rozdělena na teoreickou a praktickou část. Teoretická část probíhá formou přednášek zárověň pro všechny seminární skupiny. Vzhledem k tomu, že oborové i ročníkové spektrum studentů je dosti široké, může být tato část výuky realizována po dohodě se studenty v jinou dobu než je vymezeno v rozvrhu. Praktická část probíhá formou demonstračních cvičení. Individuální práce studentů je realizována u těch úkolů, kde to umožňují časové aspekty a vybavení laboratoře. Cvičení probíhají v max.10-ti členných sem. skupinách, podle rozvrhu.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.

Timetable of Seminar Groups

Bi3010/01: Tue 8:00–9:50 PAR
Bi3010/02: Tue 11:00–12:50 PAR
Bi3010/03: Wed 9:00–10:50 PAR
Bi3010/04: Wed 15:00–16:50 PAR
Bi3010/05: Wed 11:00–12:50 PAR
Bi3010/06: Wed 13:00–14:50 PAR

Prerequisites (in Czech)

B1050 Biological techniques || B1301 Microscopic Techniques || B2110 Microscopic techniques || B2302 Microscopical technique II. || B6660 Speciální mikroskopická technika || Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives (in Czech)

Předmětu je koncipován jako základní kurs seznamující posluchače s historií vývoje, základními konstrukčními principy a mechanismy tvorby obrazu v prozařovacím a rastrovacím elektronovém mikroskopu. Dále je pozornost věnována principům a nejdůležitějším zásadám přípravy preparátu pro oba typy mikroskopu.

Syllabus (in Czech)

• Teoretická část: Historie oboru, základní pojmy (urychlené el., rozlišovací schopnost, interakce el. s preparátem, zobrazovací systémy v el. mikroskopii, elektromagnetické čočky, vady čoček a jejich korekce) Transmisní el. mikroskop: konstrukce a princip tvorby obzazu (pružný a nepružný rozptyl, interference, kontrast Příprava preparátů pro TEM: fixace, odvodŇování, zalévání, řezání na ultramikrotomu, další metody: mrazové lámání a mrazové leptání, repliky. Rastrovací el. mikroskop: konstrukce zařízení, princip tvorby obrazu, zvětšení, rozlišení, hloubka ostrosti Příprava materiálu pro SEM: vlastnosti preparátu, sběr, fixace, odvodňování, sušení (metoda kritického bodu), pokovování (metoda iontového naprašování a vakuového napařování) Fotografická technika v el. mikroskopii Praktická část: Příprava skleněných nožů - lámání nožů na Knife-makeru, hodnocení kvality ostří, výroba vaničky na skleněný nůž Příprava formvarové fólie - potahování měděných sítěk a jejich sběr s vodní hladiny Práce na ultramikrotomu - samostatná příprava polotenkých řezů, jejich sběr na sklíčka, barvení a hodnocení. Demonstrace a samostatná obsluha TEM Demonstrace a samostatná obsluha SEM

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• DRAHOŠ, Vladimír and Armin DELONG. Praktická elektronová mikroskopie. 1. vyd. Praha: Nakladatelství Československé akademie věd, 1958, 370 s. info

Assessment methods (in Czech)

Výuka je rozdělena na teoreickou a praktickou část. Teoretická část probíhá formou přednášek zárověň pro všechny seminární skupiny. Vzhledem k tomu, že oborové i ročníkové spektrum studentů je dosti široké, může být tato část výuky realizována po dohodě se studenty v jinou dobu než je vymezeno v rozvrhu. Praktická část probíhá formou demonstračních cvičení. Individuální práce studentů je realizována u těch úkolů, kde to umožňují časové aspekty a vybavení laboratoře. Cvičení probíhají v max.10-ti členných sem. skupinách, podle rozvrhu.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

MVDr. Mgr. Monika Dušková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi3010/01: Wed 9:00–10:50 Bpm-1013, I. Hodová
Bi3010/02: Wed 11:00–12:50 Bpm-1013, I. Hodová
Bi3010/03: Wed 7:00–8:50 Bpm-1013, I. Hodová
Bi3010/04: Mon 8:00–9:50 Bpm-1013, I. Hodová
Bi3010/05: Tue 11:00–12:50 Bpm-1013, I. Hodová
Bi3010/06: Mon 10:00–11:50 Bpm-1013, I. Hodová

Prerequisites (in Czech)

B1050 Biological techniques || B1301 Microscopic Techniques || B2110 Microscopic techniques || B2302 Microscopical technique II. || B6660 Speciální mikroskopická technika || Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives (in Czech)

Předmětu je koncipován jako základní kurs seznamující posluchače s historií vývoje, základními konstrukčními principy a mechanismy tvorby obrazu v prozařovacím a rastrovacím elektronovém mikroskopu. Dále je pozornost věnována principům a nejdůležitějším zásadám přípravy preparátu pro oba typy mikroskopu.

Syllabus (in Czech)

• Teoretická část: Historie oboru, základní pojmy (urychlené el., rozlišovací schopnost, interakce el. s preparátem, zobrazovací systémy v el. mikroskopii, elektromagnetické čočky, vady čoček a jejich korekce) Transmisní el. mikroskop: konstrukce a princip tvorby obzazu (pružný a nepružný rozptyl, interference, kontrast Příprava preparátů pro TEM: fixace, odvodŇování, zalévání, řezání na ultramikrotomu, další metody: mrazové lámání a mrazové leptání, repliky. Rastrovací el. mikroskop: konstrukce zařízení, princip tvorby obrazu, zvětšení, rozlišení, hloubka ostrosti Příprava materiálu pro SEM: vlastnosti preparátu, sběr, fixace, odvodňování, sušení (metoda kritického bodu), pokovování (metoda iontového naprašování a vakuového napařování) Fotografická technika v el. mikroskopii Praktická část: Příprava skleněných nožů - lámání nožů na Knife-makeru, hodnocení kvality ostří, výroba vaničky na skleněný nůž Příprava formvarové fólie - potahování měděných sítěk a jejich sběr s vodní hladiny Práce na ultramikrotomu - samostatná příprava polotenkých řezů, jejich sběr na sklíčka, barvení a hodnocení. Demonstrace a samostatná obsluha TEM Demonstrace a samostatná obsluha SEM

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• DRAHOŠ, Vladimír and Armin DELONG. Praktická elektronová mikroskopie. 1. vyd. Praha: Nakladatelství Československé akademie věd, 1958, 370 s. info

Assessment methods (in Czech)

Výuka je rozdělena na teoreickou a praktickou část. Teoretická část probíhá formou přednášek zárověň pro všechny seminární skupiny. Vzhledem k tomu, že oborové i ročníkové spektrum studentů je dosti široké, může být tato část výuky realizována po dohodě se studenty v jinou dobu než je vymezeno v rozvrhu. Praktická část probíhá formou demonstračních cvičení. Individuální práce studentů je realizována u těch úkolů, kde to umožňují časové aspekty a vybavení laboratoře. Cvičení probíhají v max.10-ti členných sem. skupinách, podle rozvrhu.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

MVDr. Mgr. Monika Dušková, Ph.D. (lecturer)
Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

MVDr. Mgr. Monika Dušková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi3010/01: Tue 8:00–9:50 Bpz,01022, M. Dušková, I. Hodová
Bi3010/02: Fri 11:00–12:50 Bpm-1013, M. Dušková, I. Hodová
Bi3010/03: Wed 16:00–17:50 Bpm-1013, M. Dušková, I. Hodová
Bi3010/04: Mon 13:00–14:50 Bpm-1013, M. Dušková, I. Hodová
Bi3010/05: Wed 8:00–9:50 Bpm-1013, M. Dušková, I. Hodová
Bi3010/06: Wed 11:00–12:50 Bpm-1013, M. Dušková, I. Hodová
Bi3010/07: Mon 11:00–12:50 Bpm-1013, M. Dušková, I. Hodová

Prerequisites (in Czech)

B1050 Biological techniques || B1301 Microscopic Techniques || B2110 Microscopic techniques || B2302 Microscopical technique II. || B6660 Speciální mikroskopická technika || Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

The course is designed as basic course informing students about historical aspects, construction principles and mechanisms of vizualization in transmission a scanning electron microscope. Special attention is paid to the principles of the most important rules that must be complied in preparation of symplex and sections for both types of microscope.

Syllabus (in Czech)

• Teoretická část: Historie oboru, základní pojmy (urychlené el., rozlišovací schopnost, interakce el. s preparátem, zobrazovací systémy v el. mikroskopii, elektromagnetické čočky, vady čoček a jejich korekce) Transmisní el. mikroskop: konstrukce a princip tvorby obzazu (pružný a nepružný rozptyl, interference, kontrast Příprava preparátů pro TEM: fixace, odvodŇování, zalévání, řezání na ultramikrotomu, další metody: mrazové lámání a mrazové leptání, repliky. Rastrovací el. mikroskop: konstrukce zařízení, princip tvorby obrazu, zvětšení, rozlišení, hloubka ostrosti Příprava materiálu pro SEM: vlastnosti preparátu, sběr, fixace, odvodňování, sušení (metoda kritického bodu), pokovování (metoda iontového naprašování a vakuového napařování) Fotografická technika v el. mikroskopii Praktická část: Příprava skleněných nožů - lámání nožů na Knife-makeru, hodnocení kvality ostří, výroba vaničky na skleněný nůž Příprava formvarové fólie - potahování měděných sítěk a jejich sběr s vodní hladiny Práce na ultramikrotomu - samostatná příprava polotenkých řezů, jejich sběr na sklíčka, barvení a hodnocení. Demonstrace a samostatná obsluha TEM Demonstrace a samostatná obsluha SEM

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• DRAHOŠ, Vladimír and Armin DELONG. Praktická elektronová mikroskopie. 1. vyd. Praha: Nakladatelství Československé akademie věd, 1958, 370 s. info

Assessment methods (in Czech)

Výuka je rozdělena na teoreickou a praktickou část. Teoretická část probíhá formou přednášek zárověň pro všechny seminární skupiny. Vzhledem k tomu, že oborové i ročníkové spektrum studentů je dosti široké, může být tato část výuky realizována po dohodě se studenty v jinou dobu než je vymezeno v rozvrhu. Praktická část probíhá formou demonstračních cvičení. Individuální práce studentů je realizována u těch úkolů, kde to umožňují časové aspekty a vybavení laboratoře. Cvičení probíhají v max.10-ti členných sem. skupinách, podle rozvrhu.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

MVDr. Mgr. Monika Dušková, Ph.D. (lecturer)

Guaranteed by

MVDr. Mgr. Monika Dušková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi3010/01: No timetable has been entered into IS. M. Dušková
Bi3010/02: No timetable has been entered into IS. M. Dušková
Bi3010/03: No timetable has been entered into IS. M. Dušková
Bi3010/04: No timetable has been entered into IS. M. Dušková
Bi3010/05: No timetable has been entered into IS. M. Dušková
Bi3010/06: No timetable has been entered into IS. M. Dušková
Bi3010/07: No timetable has been entered into IS. M. Dušková

Prerequisites (in Czech)

B1050 Biological techniques || B1301 Microscopic Techniques || B2110 Microscopic techniques || B2302 Microscopical technique II. || B6660 Speciální mikroskopická technika || Bi1050 Biological techniques || Bi2302 Zoology microtechnique || Bi1301 Microscopic Techniques

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives (in Czech)

Předmětu je koncipován jako základní kurs seznamující posluchače s historií vývoje, základními konstrukčními principy a mechanismy tvorby obrazu v prozařovacím a rastrovacím elektronovém mikroskopu. Dále je pozornost věnována principům a nejdůležitějším zásadám přípravy preparátu pro oba typy mikroskopu.

Syllabus (in Czech)

• Teoretická část: Historie oboru, základní pojmy (urychlené el., rozlišovací schopnost, interakce el. s preparátem, zobrazovací systémy v el. mikroskopii, elektromagnetické čočky, vady čoček a jejich korekce) Transmisní el. mikroskop: konstrukce a princip tvorby obzazu (pružný a nepružný rozptyl, interference, kontrast Příprava preparátů pro TEM: fixace, odvodŇování, zalévání, řezání na ultramikrotomu, další metody: mrazové lámání a mrazové leptání, repliky. Rastrovací el. mikroskop: konstrukce zařízení, princip tvorby obrazu, zvětšení, rozlišení, hloubka ostrosti Příprava materiálu pro SEM: vlastnosti preparátu, sběr, fixace, odvodňování, sušení (metoda kritického bodu), pokovování (metoda iontového naprašování a vakuového napařování) Fotografická technika v el. mikroskopii Praktická část: Příprava skleněných nožů - lámání nožů na Knife-makeru, hodnocení kvality ostří, výroba vaničky na skleněný nůž Příprava formvarové fólie - potahování měděných sítěk a jejich sběr s vodní hladiny Práce na ultramikrotomu - samostatná příprava polotenkých řezů, jejich sběr na sklíčka, barvení a hodnocení. Demonstrace a samostatná obsluha TEM Demonstrace a samostatná obsluha SEM

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• DRAHOŠ, Vladimír and Armin DELONG. Praktická elektronová mikroskopie. 1. vyd. Praha: Nakladatelství Československé akademie věd, 1958, 370 s. info

Assessment methods (in Czech)

Výuka je rozdělena na teoreickou a praktickou část. Teoretická část probíhá formou přednášek zárověň pro všechny seminární skupiny. Vzhledem k tomu, že oborové i ročníkové spektrum studentů je dosti široké, může být tato část výuky realizována po dohodě se studenty v jinou dobu než je vymezeno v rozvrhu. Praktická část probíhá formou demonstračních cvičení. Individuální práce studentů je realizována u těch úkolů, kde to umožňují časové aspekty a vybavení laboratoře. Cvičení probíhají v max.10-ti členných sem. skupinách, podle rozvrhu.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

MVDr. Mgr. Monika Dušková, Ph.D. (lecturer)

Guaranteed by

MVDr. Mgr. Monika Dušková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi3010/01: No timetable has been entered into IS. M. Dušková
Bi3010/02: No timetable has been entered into IS. M. Dušková
Bi3010/03: No timetable has been entered into IS. M. Dušková
Bi3010/04: No timetable has been entered into IS. M. Dušková

Prerequisites (in Czech)

B1050 Biological techniques && B1301 Microscopic Techniques && B2110 Microscopic techniques && B2302 Microscopical technique II. && B6660 Speciální mikroskopická technika

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Cellular and Molecular Diagnostics (programme PřF, B-BI)
• Ecotoxicology (programme PřF, M-BI)
• Ecotoxicology (programme PřF, N-BI)
• General Biology (programme PřF, B-BI, specialization Microbiology)

Course objectives (in Czech)

Předmětu je koncipován jako základní kurs seznamující posluchače s historií vývoje, základními konstrukčními principy a mechanismy tvorby obrazu v prozařovacím a rastrovacím elektronovém mikroskopu. Dále je pozornost věnována principům a nejdůležitějším zásadám přípravy preparátu pro oba typy mikroskopu.

Syllabus (in Czech)

• Teoretická část: Historie oboru, základní pojmy (urychlené el., rozlišovací schopnost, interakce el. s preparátem, zobrazovací systémy v el. mikroskopii, elektromagnetické čočky, vady čoček a jejich korekce) Transmisní el. mikroskop: konstrukce a princip tvorby obzazu (pružný a nepružný rozptyl, interference, kontrast Příprava preparátů pro TEM: fixace, odvodŇování, zalévání, řezání na ultramikrotomu, další metody: mrazové lámání a mrazové leptání, repliky. Rastrovací el. mikroskop: konstrukce zařízení, princip tvorby obrazu, zvětšení, rozlišení, hloubka ostrosti Příprava materiálu pro SEM: vlastnosti preparátu, sběr, fixace, odvodňování, sušení (metoda kritického bodu), pokovování (metoda iontového naprašování a vakuového napařování) Fotografická technika v el. mikroskopii Praktická část: Příprava skleněných nožů - lámání nožů na Knife-makeru, hodnocení kvality ostří, výroba vaničky na skleněný nůž Příprava formvarové fólie - potahování měděných sítěk a jejich sběr s vodní hladiny Práce na ultramikrotomu - samostatná příprava polotenkých řezů, jejich sběr na sklíčka, barvení a hodnocení. Demonstrace a samostatná obsluha TEM Demonstrace a samostatná obsluha SEM

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• DRAHOŠ, Vladimír and Armin DELONG. Praktická elektronová mikroskopie. 1. vyd. Praha: Nakladatelství Československé akademie věd, 1958, 370 s. info

Assessment methods (in Czech)

Výuka je rozdělena na teoreickou a praktickou část. Teoretická část probíhá formou přednášek zárověň pro všechny seminární skupiny. Vzhledem k tomu, že oborové i ročníkové spektrum studentů je dosti široké, může být tato část výuky realizována po dohodě se studenty v jinou dobu než je vymezeno v rozvrhu. Praktická část probíhá formou demonstračních cvičení. Individuální práce studentů je realizována u těch úkolů, kde to umožňují časové aspekty a vybavení laboratoře. Cvičení probíhají v max.10-ti členných sem. skupinách, podle rozvrhu.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
Information on course enrolment limitations: souhlas

Bi3010 Electron microscopy

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity

0/2/0. 3 credit(s). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Animal System and Evolution || Bi2030 System evol. higher plants || Bi2090 System evolution vertebrates || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe to the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain of the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledges to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electronmicroscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

colloquium

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 3 credit(s). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Animal System and Evolution || Bi2030 System evol. higher plants || Bi2090 System evolution vertebrates || SOUHLAS)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe to the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain of the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledges to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.

Syllabus

• The theoretical part:
• an introduction to electron microscopy
• transmission electron microscope
• preparative methods for TEM
• scanning electron microscope
• preparative methods for SEM
• electronmicroscopic cytology
• The practical part:
• preparing glass knives
• preparing grids for ultrathin sections
• cutting and staining of semi-thin sections
• preparing samples for SEM
• individual routine operation of the electron microscope

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info

Teaching methods

theoretical lectures, laboratory practice, individual routine operation of the electron microscope

Assessment methods

colloquium

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech

Bi3010 Electron microscopy

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Iveta Hodová, Ph.D. (lecturer)

Guaranteed by

Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.

Prerequisites (in Czech)

Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives (in Czech)

Předmětu je koncipován jako základní kurs seznamující posluchače s historií vývoje, základními konstrukčními principy a mechanismy tvorby obrazu v prozařovacím a rastrovacím elektronovém mikroskopu. Dále je pozornost věnována principům a nejdůležitějším zásadám přípravy preparátu pro oba typy mikroskopu.

Syllabus (in Czech)

• Teoretická část: Historie oboru, základní pojmy (urychlené el., rozlišovací schopnost, interakce el. s preparátem, zobrazovací systémy v el. mikroskopii, elektromagnetické čočky, vady čoček a jejich korekce) Transmisní el. mikroskop: konstrukce a princip tvorby obzazu (pružný a nepružný rozptyl, interference, kontrast Příprava preparátů pro TEM: fixace, odvodŇování, zalévání, řezání na ultramikrotomu, další metody: mrazové lámání a mrazové leptání, repliky. Rastrovací el. mikroskop: konstrukce zařízení, princip tvorby obrazu, zvětšení, rozlišení, hloubka ostrosti Příprava materiálu pro SEM: vlastnosti preparátu, sběr, fixace, odvodňování, sušení (metoda kritického bodu), pokovování (metoda iontového naprašování a vakuového napařování) Fotografická technika v el. mikroskopii Praktická část: Příprava skleněných nožů - lámání nožů na Knife-makeru, hodnocení kvality ostří, výroba vaničky na skleněný nůž Příprava formvarové fólie - potahování měděných sítěk a jejich sběr s vodní hladiny Práce na ultramikrotomu - samostatná příprava polotenkých řezů, jejich sběr na sklíčka, barvení a hodnocení. Demonstrace a samostatná obsluha TEM Demonstrace a samostatná obsluha SEM

Literature

• KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
• DRAHOŠ, Vladimír and Armin DELONG. Praktická elektronová mikroskopie. 1. vyd. Praha: Nakladatelství Československé akademie věd, 1958, 370 s. info

Assessment methods (in Czech)

Výuka je rozdělena na teoreickou a praktickou část. Teoretická část probíhá formou přednášek zárověň pro všechny seminární skupiny. Vzhledem k tomu, že oborové i ročníkové spektrum studentů je dosti široké, může být tato část výuky realizována po dohodě se studenty v jinou dobu než je vymezeno v rozvrhu. Praktická část probíhá formou demonstračních cvičení. Individuální práce studentů je realizována u těch úkolů, kde to umožňují časové aspekty a vybavení laboratoře. Cvičení probíhají v max.10-ti členných sem. skupinách, podle rozvrhu.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

• Enrolment Statistics (recent)