Bi8360 Molecular diagnostics of microorganisms

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Mgr. Lenka Kosečková Micenková, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
( Bi4020 Molecular biology || Bi4010 Essential molecular biology ) && ( Bi2050 General Microbiology || Bi2060 Basic microbiology || Bi4090 General microbiology )
Basic molecular biology, structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techniques commonly used for molecular diagnostics of microorganisms. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular diagnostics methods is emphasized.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and make reasoned decisions about choosing the right methodological approaches to identification, typing and address specific biological phenomena of microbes.
Syllabus
  • 1. Introduction to the study. Significance of microbial diagnostics in medicine and industry. History of microbial diagnostics. 2. Genome structure of the main groups of microorganisms (bacteria, viruses, fungi). Genetic polymorphisms of the genomes. 3. Molecular taxonomy and molecular epidemiology. Direct and indirect diagnostic techniques. Chemotaxonomy methods. 4. Typing methods without DNA amplification. 5. Indirect diagnostic techniques with DNA amplification. 6. Direct diagnostic techniques. Sequence typing, multilocus sequence typing. 7. Data analysis, statistical methods. Classification and cluster analysis. Phylogenetic analysis. 8. Detection of antimicrobial resistance determinants. 9. Methodical approaches for molecular epidemiology and taxonomy of the most important viruses (bacterial DNA-viruses (bacteriophages); Herpesviruses, adenoviruses, RNA-viruses, Leviviruses and Lentiviruses). 10. Diagnostics and genotypic methods of analysis DNA and RNA viral genomes (RFLP, SSCP,PCR-RFLP analysis, sequencing, methods HMA (heteroduplex mobility assay) a HTA (heteroduplex tracking assay). Molecular analysis of segmented genomes of RNA viruses. 11. Genomics and DNA variation, determination of susceptibility and outcomes in microbial diseases. 12. Laboratory controls and standards, molecular test validation, monitoring and quality control. Good laboratory practice.
Literature
    recommended literature
  • Molecular microbiology : diagnostic principles and practice. Edited by David H. Persing. 2nd ed. Washington, DC: ASM Press, 2011, xvi, 936. ISBN 9781555814977. info
  • PCR Protocols for Emerging Infectious Diseases :a supplement to Diagnostic molecular microbiology : principles and applications. Edited by David H. Persing. Washington: ASM Press, 1996, xii, 180 s. ISBN 1-55581-108-6. info
  • PERSING, D.H., T.F. SMITH, F.C. TENOVER and T.J. WHITE. Diagnostic molecular microbiology. Washington, D.C.: ASM Press, 1993, 642 pp. ISBN 1-55581-056-X. info
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
General note: Předmět se doporučuje zapsat v 2. nebo 4. semestru.
The course is also listed under the following terms Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.
  • Enrolment Statistics (Spring 2025, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2025/Bi8360