C5990 Applied enzymology

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
In-person direct teaching
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.

C5990 Applied enzymology

Faculty of Science
Spring 2024
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Spring 2023
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Spring 2022
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Spring 2021
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 1. 3. to Fri 14. 5. Thu 10:00–11:50 online_BCH3
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Spring 2020
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Spring 2019
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Thu 10:00–11:50 C05/114
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
spring 2018
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Thu 10:00–11:50 C05/114
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Spring 2017
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 20. 2. to Mon 22. 5. Thu 10:00–11:50 C05/114
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Spring 2016
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Wed 11:00–12:50 C05/114
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Spring 2015
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Wed 13:00–14:50 C05/114
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2013
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Wed 14:00–15:50 C05/114
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2012
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 13:00–14:50 C05/114
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
lectures, oral examination, alternatively colloquium
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2011
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Timetable
Thu 10:00–11:50 C05/114
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
lectures, oral examination, alternatively colloquium
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2010
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Timetable
Wed 14:00–15:50 C05/114
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
lectures, oral examination, alternatively colloquium
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2009
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Timetable
Wed 15:00–16:50 C05/114
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
lectures, oral examination, alternatively colloquium
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Timetable
Thu 13:00–14:50 C05/107
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Assessment methods
lectures, oral examination, alternatively colloquium
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Aplikovaná enzymologie

Faculty of Science
Autumn 2007
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Assessment methods (in Czech)
ústní zkouška nebo kolokvium
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Seminář ke cvičení C5985

Faculty of Science
Spring 2007
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Dana Modráčková (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Příhoda, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Tue 8:00–9:50 -1020
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Aplikovaná enzymologie

Faculty of Science
Autumn 2006
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Assessment methods (in Czech)
ústní zkouška nebo kolokvium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Seminář ke cvičení C5985

Faculty of Science
Spring 2006
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Dana Modráčková (seminar tutor)
Guaranteed by
Mgr. Dana Modráčková
Department of Chemistry – Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Aplikovaná enzymologie

Faculty of Science
Autumn 2005
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Assessment methods (in Czech)
ústní zkouška nebo kolokvium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Aplikovaná enzymologie

Faculty of Science
Autumn 2004
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Assessment methods (in Czech)
ústní zkouška nebo kolokvium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Aplikovaná enzymologie

Faculty of Science
Autumn 2003
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Assessment methods (in Czech)
ústní zkouška nebo kolokvium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Aplikovaná enzymologie

Faculty of Science
Autumn 2002
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Initial information on enzymes, basic definitions, history. 2. Enzyme aktivity, units, methods of measurement, suitable substrates. 3. Isolation of enzymes, purification procedures, characterization. 4. Mechanisms of enzyme catalysis. 5. Kinetics of enzyme reaction with substrate, Michaelis-Menten equation, kinetic parameters, vmax (vlim), Km a vmax / Km. 5. Multisubstrate reactions, classification and kinetics, determination of mechanism. 6. Inhibition, reversible and irreversible inhibitors, methods of evaluation. 7. Software for enzyme kinetics. EnzLab, Gepasi. 8. Effect of external factors on enzyme reactions, pH, temperature, viscosity. 9. Cooperation effects, allosteric enzymes, Hill equation. 10. Imobilized enzymes, enzyme reactors. 11. Bioanalytical applications of enzymes. 12. Application of enzymes in immunochemical techniques. 13. Industrial applications of enzymes.
Assessment methods (in Czech)
ústní zkouška nebo kolokvium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Aplikovaná enzymologie

Faculty of Science
Autumn 2001
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors.
Assessment methods (in Czech)
ústní zkouška nebo kolokvium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Aplikovaná enzymologie

Faculty of Science
Autumn 2000
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors.
Assessment methods (in Czech)
ústní zkouška nebo kolokvium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Aplikovaná enzymologie

Faculty of Science
Autumn 1999
Extent and Intensity
2/2/0. 5 credit(s). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2024

The course is not taught in Autumn 2024

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
In-person direct teaching
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2023

The course is not taught in Autumn 2023

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2022

The course is not taught in Autumn 2022

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
autumn 2021

The course is not taught in autumn 2021

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2020

The course is not taught in Autumn 2020

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2019

The course is not taught in Autumn 2019

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2018

The course is not taught in Autumn 2018

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
autumn 2017

The course is not taught in autumn 2017

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2016

The course is not taught in Autumn 2016

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2015

The course is not taught in Autumn 2015

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2014

The course is not taught in Autumn 2014

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Seminář ke kurzu C5985

Faculty of Science
Spring 2004

The course is not taught in Spring 2004

Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Dana Modráčková (seminar tutor)
Guaranteed by
Mgr. Dana Modráčková
Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is offered to students of any study field.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2011 - acreditation

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
lectures, oral examination, alternatively colloquium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Applied enzymology

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
lectures, oral examination, alternatively colloquium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Seminář ke cvičení C5985

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
ak. mal. Zdenka Kuželová (seminar tutor)
Mgr. Dana Modráčková (seminar tutor)
RNDr. Alois Orlita, CSc. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Příhoda, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C5990 Aplikovaná enzymologie

Faculty of Science
Autumn 2007 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Assessment methods (in Czech)
ústní zkouška nebo kolokvium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (recent)