Bi6120 Plant Cell, Tissue, and Organ Culture

Faculty of Science
Spring 2008
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
RNDr. Jaroslava Dubová, CSc. (lecturer)
Guaranteed by
RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová
Timetable
Tue 8:00–9:50 C02/211
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Historical Background, Conditions of in vitro Culture. Plant Hormones and Growth Regulators. In vitro Vegetative Multiplication, Types of Regeneration, Thermotherapy. Callus Culture. Suspension Cell Culture, Direct and Indirect Somatic Embryogenesis. Test-tube Pollination, Fertilisation in vitro. Embryo Culture. Genetic Manipulation in vitro: Androgenesis (Anther Culture, Microspore and Pollen Culture), Gynogenesis (Ovary and Ovule Culture), Dihaploidization. Direct and Indirect Methods of Plant Genom Transformation. Protoplast Culture, Isolation and Fusion of Protoplasts, Somatic Hybridisation. Cryopreservation and Gene Banking. Secondary metabolites and Biotechnology.
Syllabus
  • 1. Conditions of in vitro Culture, Laboratory Design. 2. Media Preparation and Sterilization. 3. In vitro Vegetative Propagation: Primary Culture Initiation (Micropropagation by Proliferation of Axillary Buds, Adventitious shoot Proliferation), Subculture. 4. Callus Culture: Initiation of Daucus Callogenesis, Organogenesis from Callus. 5. Meristem Culture: Apical and Axillary Meristem Isolation and Culture. 6. Test-tube Pollination/ Fertilisation in vitro: Sterile Pollination of Isolated Placentae with Ovules. 7. Embryo Culture: Isolation and Culture of Young and Mature Zygotic Embryos. 8. Somatic Embryogenesis: Direct and Indirect Somatic Embryogenesis. 9. Androgenesis: Initiation of Anther Culture. 10. Plant Genome Transformation: Disc Method of Agrobacterium-Mediated Transformation. 11. Cryopreservation: Alginate Encapsulation/Dehydration Method for Meristem Cryopreservation. 12. Fungi Culture: Suspension Culture of Wood-Destroying Fungi.
Literature
  • KOVÁČ, Jaroslav. Explantátové kultury rostlin. 1. vyd. Ústí nad Labem: Univerzita Jana Evangelisty Purkyně, fakulta pedagogická, 1992, 146 s. ISBN 80-7044-036-8. info
  • ŠEBÁNEK, Jiří and Zdeněk SLADKÝ. Biotechnologie rostlinných explantátů. 1. vyd. Brno: Vysoká škola zemědělská, 1988, 100 s. info
  • PROCHÁZKA, Stanislav and Jiří ŠEBÁNEK. Regulátory rostlinného růstu. Vyd. 1. Praha: Academia, 1997, 395 s. ISBN 8020005978. info
  • NOVÁK, František Jindřich. Explantátové kultury a jejich využití ve šlechtění rostlin. 1. vyd. Praha: Academia, 1990, 208 s. ISBN 8020003444. info
  • SEMAN, Ivan. Biotechnologické metódy v šl'achtení pol'ných plodín. 1. vyd. Bratislava: Príroda, 1990, 271 s. ISBN 80-07-00237-5. info
  • GAMBORG, O. L. and G. C PHILLIPS. Plant Cell, Tissue and Organ Culture, Fundamental Methods (Plant Cell, Tissue and Organ Culture). Berlin, Heidelberg: Springer, 1995, 385 pp. info
  • PIERIK, R. L. M. In vitro culture of higher plants. Dordrecht: Martinus Nijhoff Publishers, 1987. ISBN 90-247-3531-9. info
  • In vitro embryogenesis in plants. Edited by Trevor A. Thorpe. Dordrecht: Kluwer Academic Publishers, 1995, ix, 558 s. ISBN 0-7923-3149-4. info
  • ONDŘEJ, Miloš. Genové inženýrství kulturních rostlin. 1. vyd. Praha: Academia, 1992, 232 s. ISBN 80-200-0310-X. info
  • ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2008, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2008/Bi6120