Bi6120 Plant Cell, Tissue, and Organ Culture

Faculty of Science
Spring 2009
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
RNDr. Jaroslava Dubová, CSc. (lecturer)
Guaranteed by
RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Věra Faronová
Timetable
Tue 12:00–13:50 C02/211
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Main objectives of this course can be summarized as follows: to understand the basic conditions of plant cell, tissue and organ culture in vitro; to learn the history and development of this cultures until today; to analyze the impact of different type of culture method to the plant genotype preservation and/or change; to know application of these methods for plant multiplication, breeding and production.
Syllabus
  • Historical Background, Conditions of in vitro Culture, Laboratory Design.
  • Media Composition and Preparation. Plant Hormones and Growth Regulators.
  • In vitro Vegetative Propagation: Micropropagation by Proliferation of Axillary Buds, Adventitious Shoot Proliferation; Initiation of Primary Culture, Subculture.
  • Callus Culture: Callus Initiation and Maintenance, Subculture, Organogenesis from Callus.
  • Meristem Culture: Apical and Axillary Meristem Isolation and Culture.
  • Test-tube Pollination/ Fertilization in vitro: Sterile Pollination of Isolated Placentae with Ovules. Fertiliation of Isolated Ovules and/or Izolated Gametes.
  • Embryo Culture: Isolation and Culture of Young and Mature Zygotic Embryos.
  • Somatic Embryogenesis: Direct and Indirect Somatic Embryogenesis.
  • Ploidy reduction: Androgenesis: Initiation of Anther or Microspore Culture,Chromosome Doubling.
  • Plant Genome Transformation (GMO): Direct (Electroporation, Vacuum Infiltration, Biolistic Method and Indirect Methods (Disc Method of Agrobacterium-Mediated Transformation).
  • Cryopreservation: Rapid and Slow Freezing (Vitrification, Alginate Encapsulation/Dehydration Method).
  • Fungi Culture in vitro: Suspension Culture of Wood-Destroying Fungi.
  • Indexing and Certification of in vitro Cultures
Literature
  • KOVÁČ, Jaroslav. Explantátové kultury rostlin. 1. vyd. Ústí nad Labem: Univerzita Jana Evangelisty Purkyně, fakulta pedagogická, 1992, 146 s. ISBN 80-7044-036-8. info
  • ŠEBÁNEK, Jiří and Zdeněk SLADKÝ. Biotechnologie rostlinných explantátů. 1. vyd. Brno: Vysoká škola zemědělská, 1988, 100 s. info
  • PROCHÁZKA, Stanislav and Jiří ŠEBÁNEK. Regulátory rostlinného růstu. Vyd. 1. Praha: Academia, 1997, 395 s. ISBN 8020005978. info
  • NOVÁK, František Jindřich. Explantátové kultury a jejich využití ve šlechtění rostlin. 1. vyd. Praha: Academia, 1990, 208 s. ISBN 8020003444. info
  • SEMAN, Ivan. Biotechnologické metódy v šl'achtení pol'ných plodín. 1. vyd. Bratislava: Príroda, 1990, 271 s. ISBN 80-07-00237-5. info
  • GAMBORG, O. L. and G. C PHILLIPS. Plant Cell, Tissue and Organ Culture, Fundamental Methods (Plant Cell, Tissue and Organ Culture). Berlin, Heidelberg: Springer, 1995, 385 pp. info
  • PIERIK, R. L. M. In vitro culture of higher plants. Dordrecht: Martinus Nijhoff Publishers, 1987. ISBN 90-247-3531-9. info
  • In vitro embryogenesis in plants. Edited by Trevor A. Thorpe. Dordrecht: Kluwer Academic Publishers, 1995, ix, 558 s. ISBN 0-7923-3149-4. info
  • ONDŘEJ, Miloš. Genové inženýrství kulturních rostlin. 1. vyd. Praha: Academia, 1992, 232 s. ISBN 80-200-0310-X. info
  • ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
  • SLATER, Adrian, Nigel W. SCOTT and Mark R. FOWLER. Plant biotechnology : the genetic manipulation of plants. 1st publ. New York: Oxford University Press, 2003, xxii, 346. ISBN 0199254680. info
  • Phytohormones in plant biotechnology and agriculture : proceedings of the NATO-Russia Workshop held in Moscow, 12-16 May 2002. Edited by Ivana Macháčková - Georgy A. Romanov. Dodrecht: Kluwer Academic Publishers, 2003, xiv, 265. ISBN 1402017235. info
  • Plant biotechnology : current and future applications of genetically modified crops. Edited by Nigel G. Halford. Chichester: John Wiley & Sons, 2006, xii, 303. ISBN 0470021810. info
Assessment methods
written test or oral exam
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2009, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2009/Bi6120