PřF:Bi6120 Plant Tissue and Organ Culture - Course Information
Bi6120 Plant Cell, Tissue, and Organ Culture
Faculty of ScienceSpring 2012
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 8:00–9:50 C13/332
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- At the end of the course students should be able to:
describe basic principles of plant cell, tissue and organ culture in vitro;
explain the history and development of this cultures until today;
analyze the impact of different types of culture method on the plant genotype preservation and/or change;
propose in vitro methods suitable for plant multiplication, breeding and production. - Syllabus
- Historical Background, Conditions of in vitro Culture, Laboratory Design.
- Media Composition and Preparation. Plant Hormones and Growth Regulators.
- In vitro Vegetative Propagation: Micropropagation by Proliferation of Axillary Buds, Adventitious Shoot Proliferation; Initiation of Primary Culture, Subculture.
- Callus Culture: Callus Initiation and Maintenance, Subculture, Organogenesis from Callus.
- Meristem Culture: Apical and Axillary Meristem Isolation and Culture.
- Test-tube Pollination/ Fertilization in vitro: Sterile Pollination of Isolated Placentae with Ovules. Fertiliation of Isolated Ovules and/or Izolated Gametes.
- Embryo Culture: Isolation and Culture of Young and Mature Zygotic Embryos.
- Somatic Embryogenesis: Direct and Indirect Somatic Embryogenesis.
- Ploidy reduction: Androgenesis: Initiation of Anther or Microspore Culture,Chromosome Doubling.
- Plant Genome Transformation (GMO): Direct (Electroporation, Vacuum Infiltration, Biolistic Method and Indirect Methods (Disc Method of Agrobacterium-Mediated Transformation).
- Cryopreservation: Rapid and Slow Freezing (Vitrification, Alginate Encapsulation/Dehydration Method).
- Fungi Culture in vitro: Suspension Culture of Wood-Destroying Fungi.
- Indexing and Certification of in vitro Cultures
- Literature
- KOVÁČ, Jaroslav. Explantátové kultury rostlin. 1. vyd. Ústí nad Labem: Univerzita Jana Evangelisty Purkyně, fakulta pedagogická, 1992, 146 s. ISBN 80-7044-036-8. info
- ŠEBÁNEK, Jiří and Zdeněk SLADKÝ. Biotechnologie rostlinných explantátů. 1. vyd. Brno: Vysoká škola zemědělská, 1988, 100 s. info
- PROCHÁZKA, Stanislav and Jiří ŠEBÁNEK. Regulátory rostlinného růstu. Vyd. 1. Praha: Academia, 1997, 395 s. ISBN 8020005978. info
- NOVÁK, František Jindřich. Explantátové kultury a jejich využití ve šlechtění rostlin. 1. vyd. Praha: Academia, 1990, 208 s. ISBN 8020003444. info
- SEMAN, Ivan. Biotechnologické metódy v šl'achtení pol'ných plodín. 1. vyd. Bratislava: Príroda, 1990, 271 s. ISBN 80-07-00237-5. info
- GAMBORG, O. L. and G. C PHILLIPS. Plant Cell, Tissue and Organ Culture, Fundamental Methods (Plant Cell, Tissue and Organ Culture). Berlin, Heidelberg: Springer, 1995, 385 pp. info
- PIERIK, R. L. M. In vitro culture of higher plants. Dordrecht: Martinus Nijhoff Publishers, 1987. ISBN 90-247-3531-9. info
- In vitro embryogenesis in plants. Edited by Trevor A. Thorpe. Dordrecht: Kluwer Academic Publishers, 1995, ix, 558 s. ISBN 0-7923-3149-4. info
- ONDŘEJ, Miloš. Genové inženýrství kulturních rostlin. 1. vyd. Praha: Academia, 1992, 232 s. ISBN 80-200-0310-X. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- SLATER, Adrian, Nigel W. SCOTT and Mark R. FOWLER. Plant biotechnology : the genetic manipulation of plants. 1st publ. New York: Oxford University Press, 2003, xxii, 346. ISBN 0199254680. info
- Phytohormones in plant biotechnology and agriculture : proceedings of the NATO-Russia Workshop held in Moscow, 12-16 May 2002. Edited by Ivana Macháčková - Georgy A. Romanov. Dodrecht: Kluwer Academic Publishers, 2003, xiv, 265. ISBN 1402017235. info
- Plant biotechnology : current and future applications of genetically modified crops. Edited by Nigel G. Halford. Chichester: John Wiley & Sons, 2006, xii, 303. ISBN 0470021810. info
- Teaching methods
- Lectures.
- Assessment methods
- Written test or oral exam.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Enrolment Statistics (Spring 2012, recent)
- Permalink: https://is.muni.cz/course/sci/spring2012/Bi6120