C8113 Biotechnology methods

Faculty of Science
Spring 2021
Extent and Intensity
0/0/5. 5 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Jiří Kučera, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main aim of the course is to acquire basic methods used in biotechnology. In practical exercises, students will be familiarized with the procedures typical for developing and realizing the biotechnology process in a bioreactor with subsequent purification of the product.
Learning outcomes
Students will know the basic principles of applying selected methods used in biotechnology and use different methods for further experimental work in biotechnology and related fields.
Syllabus
  • Bioreactor preparation. Feed-batch cultivation of recombinant microbial strain with subsequent production of the target protein. Monitoring of bioprocess parameters such as aeration, temperature, biomass, substrate, and product concentrations. Biomass separation and cell lysate preparation. Purification of the target recombinant protein by fast protein liquid chromatography.
Literature
    recommended literature
  • Methods in biotechnology. Edited by Hans-Peter Schmauder. London: Taylor & Francis, 1997, ix, 257 s. ISBN 0-7484-0429-5. info
  • POSTEN, Clemens. Integrated bioprocess engineering. Berlin: De Gruyter, 2018, xi, 344. ISBN 9783110315387. info
  • Protein purification : principles, high resolution methods, and applications. Edited by Jan-Christer Janson. 3rd ed. Hoboken, N.J.: John Wiley & Sons, 2011, xiv, 517. ISBN 9780471746614. info
Teaching methods
Individually supervised laboratory course.
Assessment methods
Compulsory participation throughout the block exercise. Completion of credit based on participation and processing of protocols.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is taught in blocks.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2021, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2021/C8113