Bi6120 Plant Cell, Tissue, and Organ Culture

Faculty of Science
Spring 2022
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Hana Cempírková, Ph.D. (lecturer)
Guaranteed by
Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 8:00–9:50 C13/332
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to:
describe basic principles of plant cell, tissue and organ culture in vitro;
explain the history and development of this cultures until today;
analyze the impact of different types of culture method on the plant genotype preservation and/or change;
propose in vitro methods suitable for plant multiplication, breeding and production.
Learning outcomes
At the end of the course students should be able to:
describe basic principles of plant cell, tissue and organ culture in vitro;
explain the history and development of this cultures until today;
analyze the impact of different types of culture method on the plant genotype preservation and/or change;
propose in vitro methods suitable for plant multiplication, breeding and production.
Syllabus
  • 1. Historical Background, Conditions of in vitro Culture, Laboratory Design.
  • 2. Media Composition and Preparation. Plant Hormones and Growth Regulators.
  • 3. In vitro Vegetative Propagation: Micropropagation by Proliferation of Axillary Buds, Adventitious Shoot Proliferation; Initiation of Primary Culture, Subculture.
  • 4. Callus Culture: Callus Initiation and Maintenance, Subculture, Organogenesis from Callus.
  • 5. Meristem Culture: Apical and Axillary Meristem Isolation and Culture.
  • 6. Test-tube Pollination/ Fertilization in vitro: Sterile Pollination of Isolated Placentae with Ovules. Fertiliation of Isolated Ovules and/or Isolated Gametes.
  • 7. Embryo Culture: Isolation and Culture of Young and Mature Zygotic Embryos.
  • 8. Somatic Embryogenesis: Direct and Indirect Somatic Embryogenesis.
  • 9. Ploidy reduction: Androgenesis: Initiation of Anther or Microspore Culture,Chromosome Doubling.
  • 10. Plant Genome Transformation (GMO): Direct (Electroporation, Vacuum Infiltration, Biolistic Method and Indirect Methods (Disc Method of Agrobacterium-Mediated Transformation).
  • 11. Cryopreservation: Rapid and Slow Freezing (Vitrification, Alginate Encapsulation/Dehydration Method).
  • 12. Fungi Culture in vitro: Suspension Culture of Wood-Destroying Fungi.
  • 13. Secondary metabolites.
  • 14. Indexing and Certification of in vitro Cultures
Literature
  • SMITH, Roberta H. Plant tissue culture : techniques and experiments. Third edition. Amsterdam: Elsevier, 2013, xvi, 188. ISBN 9780124159204. info
  • KOVÁČ, Jaroslav. Explantátové kultury rostlin. 1. vyd. Ústí nad Labem: Univerzita Jana Evangelisty Purkyně, fakulta pedagogická, 1992, 146 s. ISBN 80-7044-036-8. info
  • GAMBORG, O. L. and G. C PHILLIPS. Plant Cell, Tissue and Organ Culture, Fundamental Methods (Plant Cell, Tissue and Organ Culture). Berlin, Heidelberg: Springer, 1995, 385 pp. info
  • PROCHÁZKA, Stanislav and Jiří ŠEBÁNEK. Regulátory rostlinného růstu. Vyd. 1. Praha: Academia, 1997, 395 s. ISBN 8020005978. info
  • SLATER, Adrian, Nigel W. SCOTT and Mark R. FOWLER. Plant biotechnology : the genetic manipulation of plants. 1st publ. New York: Oxford University Press, 2003, xxii, 346. ISBN 0199254680. info
  • Plant biotechnology : current and future applications of genetically modified crops. Edited by Nigel G. Halford. Chichester: John Wiley & Sons, 2006, xii, 303. ISBN 0470021810. info
  • Phytohormones in plant biotechnology and agriculture : proceedings of the NATO-Russia Workshop held in Moscow, 12-16 May 2002. Edited by Ivana Macháčková - Georgy A. Romanov. Dodrecht: Kluwer Academic Publishers, 2003, xiv, 265. ISBN 1402017235. info
  • In vitro embryogenesis in plants. Edited by Trevor A. Thorpe. Dordrecht: Kluwer Academic Publishers, 1995, ix, 558 s. ISBN 0-7923-3149-4. info
Teaching methods
Lectures.
Assessment methods
Written test or oral exam.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, Spring 2019, Spring 2021, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2022/Bi6120