VSBC0221p Biochemistry I - lecture

Faculty of Medicine
Spring 2009
Extent and Intensity
2/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Jiří Dostál, CSc. (lecturer)
prof. MUDr. Vladimír Palyza, CSc. (lecturer)
doc. MUDr. Jaromír Sláma, CSc. (lecturer)
prof. RNDr. Eva Táborská, CSc. (lecturer)
Mgr. Milena Matejovičová, Ph.D. (seminar tutor)
Ing. Jitka Melounová, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
PharmDr. Lenka Adámková, Ph.D. (assistant)
Mgr. Jan Alán (assistant)
RNDr. Hana Bochořáková (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
Mgr. Martin Klepárník, Ph.D. (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Lenka Nerudová (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
doc. RNDr. Josef Tomandl, Ph.D. (alternate examiner)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
VSBC0221p/NUT: Wed 11:30–13:30 Kinosál N02901
VSBC0221p/01: Wed 11:30–13:30 Kinosál N02901
VSBC0221p/02: Wed 7:30–9:20 P31 Posluchárna A. I. Bláhy
Prerequisites (in Czech)
VSLC011s Med.Chem.-seminary && VSLC011c Medical Chemistry -p
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to obtain knowledge on essential metabolic processes on the cell level. The graduate is acquainted with properties and function of enzymes, is oriented in catabolic and anabolic pathways of metabolism of saccharides, lipids and proteins and their consequences.Understands the principles of cell bioenegetics.Become familiar with the funcion of cell membranes, principle of cell compartmentation and membrane transport.Has knowledge on proteosynthesis including processes replication,transcription, translation and posttranslantion modifications.Understands the relation between the structure and function of proteins. Is acquinted with function of hemoglobine in oxygen transport.Molecular bases of some diseases are introduced. The course provides the essential knowledge for future understanding of metabolism on organe and inter-organe level and its disturbances.
Syllabus
  • Membrane structure, the assembly and recycling of membranes. Specialized structures of plasma membrane – lipid rafts, caveols, tight junctions. membrane transport. Enzymes. Characteristic features of biocatalysis, enzyme structure and function, nomenclature and classification of enzymes. Enzyme cofactors, review of structures and functions. Mechanisms of enzyme action. Kinetics of enzyme catalyzed reactions. Assays of enzyme activity, the conditions used. Factors affecting catalytic activity of enzymes, types of enzyme inhibition. Metabolism: basic concepts and design. Biological oxidations, generation of high-energy compounds. Saccharide metabolism: the glycolytic pathway and aerobic decarboxylation of pyruvate. Gluconeogenesis. Glycogen biosynthesis and breakdown. The pentose phosphate pathway. The glucuronate pathway. Interconversions of monosaccharides and of their derivatives. Protein and amino acid metabolism. The common reactions in amino acid degradation. The ureosynthetic cycle. Metabolic breakdown of individual amino acids. Biosynthesis and breakdown of fatty acids, ketogenesis. Synthesis of triacylglycerols. Metabolism of phospholipids and glycolipids. Synthesis of eicosanoids. Biosynthesis and transformations of cholesterol, biosynthesis of bile acids. Interrelationships among the major pathways involved in energy metabolism. The citric acid cycle. Synthesis of haem. Mitochondria. Oxidative phosphorylation - mitochondrial electron transport chain, synthesis of ATP. Structure of haemoglobin, structure-function relationships (the oxygen saturation curve, inducement of haemoglobin saturation and oxygen transport. Bohr effect. Normal haemoglobin types in blood, haemoglobin concentration. Other forms (glycohaemoglobin, methaemoglobin, carboxyhaemoglobin) and abnormal haemoglobins. Biosynthesis and catabolism of purine and pyrimidine nucleotides. Chromatin, DNA replication. DNA transcription. Regulation of gene expression. Protein synthesis and post-translational processing.
Literature
  • LEDVINA, Miroslav, Alena STOKLASOVÁ and Jaroslav CERMAN. Biochemie pro studující medicíny (I. a II. díl). 1st ed. Praha: Karolinum, 2004, 561 pp. ISBN 80-246-0851-0. info
  • MURRAY, R.K. and D.K. GRANNER. Harperova biochemie. 3. v ČR. Jinočany: H & H, 2001, 872 pp. ISBN 80-7319-003-6. info
  • HOLEČEK, Milan. Regulace metabolizmu cukrů, tuků, bílkovin a aminokyselin. 1st ed. Praha: Grada Publishing, 2006, 286 pp. učebnice. ISBN 80-247-1562-7. info
  • Biochemistry :a concise text for medical students. Edited by D. K. Apps. 5th ed. London: Bailliere Tindall, 1992, vii, 448 s. ISBN 0-7020-1444-3. info
  • MURRAY, R. K., D. K. GRANNER and V. W. RODWELL. Harper's Illustrated Biochemistry. 27th ed. New York: Lange, 2006, 692 pp. textbook. ISBN 0-07-147885-x. info
  • CHAMPE, P. C., R. A. HARVEY and D. R. FERRIER. Lippincott's Illustrated Reviews: Biochemistry. 3rd ed. Philadelphia: Lippincott Williams & Wilkins, 2005, 534 pp. textbook. ISBN 0-7817-2265-9. info
Assessment methods
Teaching form are lectures. Supplementary subjects are seminars VSBC021s
Student must have course-unit credit of seminar in the day of exam. Examination has writen and oral part. Writen part has 25 questions and is solved on computers in computer room of Department of Biochemistry. The second part is oral.Examination questions and other instructions you will find in the section Study materials of the course.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: zkoušku lze konat až po absolvování zkoušky z Lékařské chemie
The course is taught annually.
Information on course enrolment limitations: Přihlášení ke zkoušce je podmíněno vykonáním zkoušky z Lékařské chemie.
Listed among pre-requisites of other courses
Teacher's information
http://www.med.muni.cz/toCP1250.cs/biochem/vyuka.html
The course is also listed under the following terms Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Autumn 2008, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016.
  • Enrolment Statistics (Spring 2009, recent)
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