LF:VSBC0221p Biochemistry I -lect.

VSBC0221p Biochemistry I - lecture

Faculty of Medicine
Spring 2012

Extent and Intensity

2/0. 4 credit(s). Type of Completion: zk (examination).

Teacher(s)

Guaranteed by

prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine

Timetable

Thu 10:30–12:20 B22/116 aula

Timetable of Seminar Groups:

VSBC0221p/VLA: Wed 13:00–14:50 B11/234

Prerequisites (in Czech)

VSLC011s Med.Chem.-seminary && VSLC011c Medical Chemistry -p && ZC011 Handling chemical substances

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

The aim of the course is to obtain knowledge on essential metabolic processes on the cell level. The graduate is acquainted with properties and function of enzymes, is oriented in catabolic and anabolic pathways of metabolism of saccharides, lipids and proteins and their consequences.Understands the principles of cell bioenegetics.Become familiar with the funcion of cell membranes, principle of cell compartmentation and membrane transport.Has knowledge on proteosynthesis including processes replication,transcription, translation and posttranslantion modifications.Understands the relation between the structure and function of proteins. Is acquinted with function of hemoglobine in oxygen transport.Molecular bases of some diseases are introduced. The course provides the essential knowledge for future understanding of metabolism on organe and inter-organe level and its disturbances.

Syllabus

Membrane structure, the assembly and recycling of membranes. Specialized structures of plasma membrane – lipid rafts, caveols, tight junctions. membrane transport. Enzymes. Characteristic features of biocatalysis, enzyme structure and function, nomenclature and classification of enzymes. Enzyme cofactors, review of structures and functions. Mechanisms of enzyme action. Kinetics of enzyme catalyzed reactions. Assays of enzyme activity, the conditions used. Factors affecting catalytic activity of enzymes, types of enzyme inhibition. Metabolism: basic concepts and design. Biological oxidations, generation of high-energy compounds. Saccharide metabolism: the glycolytic pathway and aerobic decarboxylation of pyruvate. Gluconeogenesis. Glycogen biosynthesis and breakdown. The pentose phosphate pathway. The glucuronate pathway. Interconversions of monosaccharides and of their derivatives. Protein and amino acid metabolism. The common reactions in amino acid degradation. The ureosynthetic cycle. Metabolic breakdown of individual amino acids. Biosynthesis and breakdown of fatty acids, ketogenesis. Synthesis of triacylglycerols. Metabolism of phospholipids and glycolipids. Synthesis of eicosanoids. Biosynthesis and transformations of cholesterol, biosynthesis of bile acids. Interrelationships among the major pathways involved in energy metabolism. The citric acid cycle. Synthesis of haem. Mitochondria. Oxidative phosphorylation - mitochondrial electron transport chain, synthesis of ATP. Structure of haemoglobin, structure-function relationships (the oxygen saturation curve, inducement of haemoglobin saturation and oxygen transport. Bohr effect. Normal haemoglobin types in blood, haemoglobin concentration. Other forms (glycohaemoglobin, methaemoglobin, carboxyhaemoglobin) and abnormal haemoglobins. Biosynthesis and catabolism of purine and pyrimidine nucleotides. Chromatin, DNA replication. DNA transcription. Regulation of gene expression. Protein synthesis and post-translational processing.

Literature

required literature

LEDVINA, Miroslav, Alena STOKLASOVÁ and Jaroslav CERMAN. Biochemie pro studující medicíny, I. a II. díl. Druhé vydání. Praha: Karolinum, 2009, 546 pp. ISBN 978-80-246-1414-4. info

recommended literature

HOLEČEK, Milan. Regulace metabolizmu cukrů, tuků, bílkovin a aminokyselin. 1st ed. Praha: Grada Publishing, 2006, 286 pp. učebnice. ISBN 80-247-1562-7. info
HARVEY, Richard A. and Denise R. FERRIER. Biochemistry. 5th edition. Baltimore: Wolters Kluver, Lippincott Williams & Wilkins, 2011. International Edition. ISBN 978-1-60913-998-8. info

Teaching methods

Teaching form are lectures. Supplementary subjects are seminars VSBC021s

Assessment methods

Student must have course-unit credit of seminar in the day of exam. Examination has writen and oral part. Writen part has 25 questions and is solved on computers in computer room of Department of Biochemistry. The second part is oral.Examination questions and other instructions you will find in the section Study materials of the course.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
Information on completion of the course: zkoušku lze konat až po absolvování zkoušky z Lékařské chemie
The course is taught annually.
Information on course enrolment limitations: Přihlášení ke zkoušce je podmíněno vykonáním zkoušky z Lékařské chemie.

Listed among pre-requisites of other courses

Teacher's information

http://www.med.muni.cz/toCP1250.cs/biochem/vyuka.html

The course is also listed under the following terms Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Autumn 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2013, Spring 2014, Spring 2015, Spring 2016.

Enrolment Statistics (Spring 2012, recent)
Permalink: https://is.muni.cz/course/med/spring2012/VSBC0221p

LF:VSBC0221p Biochemistry I -lect. - Course Information

VSBC0221p Biochemistry I - lecture

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