PřF:Bi5599 App.Chem.and Biochem. - Course Information

Bi5599 Applied Chemistry and Biochemistry

Extent and Intensity

2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

prof. RNDr. Jan Vondráček, Ph.D. (lecturer)
RNDr. Miroslav Machala, CSc. (lecturer), MVDr. Mgr. Monika Dušková, Ph.D. (deputy)

Guaranteed by

prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Vondráček, Ph.D.

Timetable

Thu 9:00–10:50 BFU

Prerequisites (in Czech)

Bi4020 Molecular biology && C3580 Biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

Main objectives of the course are to provide students with an overview of basic methods for analysis of macromolecules (DNA, RNA, proteins, lipids); with laboratory techniques for work with low molecular weight compounds; analyses of cell populations and subcellular structures; basic principles of in vivo experiments employing genetically modified mammals. The course is taught as lecture and seminar aimed at work with primary literature, in order to understand the importance of individual techniques and to interpret both literary and experimental data. Students should actively participate in seminars.

Syllabus

• 1)Protein analysis; separation techniques; Western blotting, protein-protein interactions; protein localization; protein post-translational modifications; proteomics; manipulations of protein expression.
• 2)Basic immunochemistry techniques.
• 3)DNA isolation, separation; PCR techniques, plasmid work, transfection of eukaryotic cells.
• 4)RNA isolation, detection (Northern blotting, RT-PCR), microarrays, siRNA.
• 5)Lipids and polysacharidy; isolation, separation and identification.
• 6)Low-molecular-weight compounds - separation and analytical techniques; HPLC techniques and their modifications.
• 7)Specific cellular assays.
• 8)Fluorescennce techniques.
• 9)Aplication of in vivo mammalian models in physiology.

Literature

• Alberts et al.: Molecular Biology of the Cell, 4th ed., Garland Science, 2002
• Klouda, Moderní analytické metody, Pavel Klouda, Ostrava, 2003
• Wilson et al.: Principles and Techniques of Practical Biochemistry, 5th ed., CUP, 2000

Assessment methods

Final seminar. Each student is required to prepare a presentation about methodical approaches used in an assigned paper.

Language of instruction

Czech

Further Comments

Study Materials

Listed among pre-requisites of other courses

• Bi5599c Applied Biochemistry and Cell Biology Methods - practice
  Bi3030 && NOW(Bi5599)  

• Enrolment Statistics (Autumn 2008, recent)
  
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