PřF:Bi1301 Microscopic Techniques - Course Information

Bi1301 Microscopic Methods for the Study of Plant Structure

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Jaroslava Dubová, CSc. (seminar tutor)

Guaranteed by

RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi1301/01: Thu 10:00–11:50 C13/332, J. Dubová
Bi1301/02: Thu 12:00–13:50 C13/332, J. Dubová

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of this practical course students should be able to:
describe the historical and present construction of light microscopes;
operate light microscopes in different modes and techniques;
prepare various kinds of botanical microscopic objects;
document these objects by means of microphotography;
process the microphotographs.

Syllabus

• Introduction to the Methods for the Study of Plant Structure: Light Microscope Development, Structure and Function. Koehler Illumination.
• Replicas: Study of the Surface Structure by Light Microscope. Stoma. Subsidiary and Neighbour Cells; Stoma Types.
• Cleared Preparations: Angiosperm Embryo Development.
• Squash Methods: Cell Cycle, Mitosis, Chromosome Staining.
• Anatomical Methods: Fixation. Dehydration. Infiltration and Embedding. Paraffin Sections. Sections Staining and Mounting.
• Vital Preparations: Phase Contrast Microscopy, Nomarski DIC.
• Pollen: Differential Staining and Pollen Viability
• Fluorescence Microscopy: Primary and Secondary Fluorescence, Immunofluorescence.
• Documentation: Measurements. Photomicrography.

Literature

• HABROVÁ, Věra. Mikroskopická technika. Praha: SPN, 1990. info
• KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
• PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
• NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
• Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
• ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
• PAZOUREK, Jaroslav. Atlas anatomické stavby rostlin. Praha: Karolinum, 1992. info
• KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
• BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
• BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info

Teaching methods

Practical laboratory course.

Assessment methods

Protocols of used methods, written test, credit.

Language of instruction

Czech

Follow-Up Courses

• Bi7270 Plant Embryology

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• Bi1050 Biological techniques
  !Bi2302 && !Bi1301 && (!NOW(Bi2302)) && (!NOW(Bi1301))  

• Enrolment Statistics (Autumn 2012, recent)
  
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