Bi1050 The Study of Animal and Plant Structure for Biology Teachers

Faculty of Science
Autumn 2019
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Milan Baláž, Ph.D. (seminar tutor)
Mgr. Mária Seifertová, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Mária Seifertová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Mária Seifertová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi1050/01: Tue 10:00–11:50 D36/223, M. Baláž, M. Seifertová
Bi1050/02: Tue 12:00–13:50 D36/223, M. Baláž, M. Seifertová
Bi1050/03: Thu 14:00–15:50 D36/223, M. Baláž, M. Seifertová
Prerequisites (in Czech)
! Bi2302 Zoological microtechnique && ! Bi1301 Microscopic Techniques && (!NOW( Bi2302 Zoological microtechnique )) && (!NOW( Bi1301 Microscopic Techniques ))
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main aims in the course are a)to acquaint students with the basic techniques of light microscopy and the possibilities of processing biological material in the form of different types of prepared microscope slides; b) to learn students with basic principles of design and analysis of biological experiments; c) give students a short overview of computer-aided image analysis.
Learning outcomes
At the end of this practical course, students should be able - to use various techniques of light microscopy efficiently; - to prepare various kinds of botanical or zoological mounts; - to take and efectivelly process microphotographs; - to utilize some simple techniques of image analysis; - to conduct simple biological experiment including adequate statistical treatment of the data; - to utilize basic facts about mitosis, embryogenesis and the other biological processes in their own teaching practice.
Syllabus
  • Introduction. Light microscope: structure and function. Köhler illumination. Botanical techniques: Microrelief technique for the study of the stomata structure and density. Whole mounts of cleared preparations: development of Angiosperm (dicotyledonous plant) embryo. Squash preparations: cell cycle, mitosis, chromosome counting. Anatomical methods: sectioning (hand sections, fixation, infiltration and embedding, microtome Sections), staining. Documentation - hand drawing, microphotographs and their processing. Simple technique of image analysis - leaf area measurement. Design and statistical treatment of simple biological experiments. Zoological techniques: Native preparations. Vital staining. Whole mounts, sections, squashes, dry mounts. Fixation. Fixatives. Staining. Aqueous media. Non-aqueous mounting media.
Literature
    recommended literature
  • KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
  • PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
  • LELLÁKOVÁ, Františka. Zoologická technika. 1. vyd. Praha: Universita Karlova, 1985, 122 s. info
  • NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
  • BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
  • BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
Teaching methods
Practical training.
Assessment methods
Whole mounts must be prepared to pass the zoological part of the course. Botanical part of the course: active participation, elaboration of three protocols, written test in which 75% of correct answers is needed to pass.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/kfar/html/biologicka_technika_navody.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2024.
  • Enrolment Statistics (Autumn 2019, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2019/Bi1050