aVLBC0321p Biochemistry I - lecture

Faculty of Medicine
autumn 2023
Extent and Intensity
3/0/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jiří Dostál, CSc. (lecturer)
prof. RNDr. Eva Táborská, CSc. (lecturer)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
MUDr. Michaela Králíková, Ph.D. (assistant)
RNDr. Hana Paulová, CSc. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Mon 18. 9. to Fri 6. 10. Wed 12:00–12:50 B11/114, Mon 16. 10. to Fri 27. 10. Wed 12:00–12:50 B11/114, Mon 30. 10. to Fri 22. 12. Tue 12:00–13:50 B22/116 aula, Mon 6. 11. to Fri 22. 12. Wed 12:00–12:50 B11/114, Mon 18. 9. to Fri 20. 10. Tue 12:00–13:50 B22/116 aula; and Wed 11. 10. 12:00–12:50 B11/334, Tue 24. 10. 12:00–13:50 Kinosál N02901, Wed 1. 11. 12:00–12:50 B11/234
Prerequisites (in Czech)
aVLBI0222p Medical Biology II - lect. && aVLBF011p Biophysics -lect.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to gain knowledge about the basic metabolic processes and pathways taking place at the cellular level.
Understanding these processes is the basis for understanding metabolism at the tissue and organ level, which is the focus of the follow-up course Biochemistry II.
Learning outcomes
At the end of the course, students will:
- understand the meaning of basic chemical concepts and can apply this knowledge to describe biochemical processes in the body and the properties of body fluids.
- discuss the properties and function of enzymes.
- describe basic catabolic and anabolic pathways of carbohydrate, lipid and protein metabolism and their relationships.
- understand the principles of energy production, utilization and deposition at the cellular level.
- explain the function of cell membranes and the principle of compartmentalization at the cellular level, and the transport processes on the membrane.
- describe protein synthesis, starting with the replication and transcription, translation and post-translational modifications.
- understand the relationship between protein structure and function.
- explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
- discuss the principles of some diseases at the molecular level.
Syllabus
  • Electrolytes, ion activity. Weak acids/bases. Buffers. Bioenergetics, Gibbs energy, high-energy compounds. Redox reaction.
  • Enzymes. Characteristic features of biocatalysis. Cofactors.
  • Metabolism of nutrients. Citrate cycle. Respiratory chain. Oxidative phosphorylation.
  • Carbohydrates in nutrition. Carbohydrate digestion. Basic features of glucose metabolism. Glucose transport into cells. Glycolysis. Gluconeogenesis.
  • Glycogen. Metabolism of fructose, galactose, lactose. The pentose phosphate pathway. Glycosaminoglycans, proteoglycans.
  • Lipids and fatty acids. Surfactants. Lipophilic vitamins. Lipids in nutrition. Lipid digestion. Fatty acid metabolism. PUFA synthesis. Metabolism of ketone bodies.
  • Metabolism of triacylglycerols, glycerophospholipids, sphingolipids. Lipid peroxidation. Biosynthesis and importance of eicosanoids.
  • Metabolism of cholesterol, bile acids. Biosynthesis of calciols.
  • Amino acids. Peptides. Structure of proteins, myosin, collagen, immunoglobulins. Hemoglobin, myoglobin. Hemoglobinopathies.
  • Digestion and intracellular degradation of proteins. General features in catabolism of amino acids. Ammonia metabolism and toxicity.
  • Catabolism of amino acids, congenital disorders of amino acid metabolism. Synthesis of non-essential amino acids.
  • Conversions of amino acids into low molecular weight products. Heme synthesis, porphyria.
  • Metabolism of purine/pyrimidine nucleotides.
  • Structure of DNA, RNA. Replication and corrections. Transcription and its regulation, post-transcriptional modifications.
  • Protein synthesis. Folding, transport and sorting of proteins in the cell. Post-translational modification.
  • Biosynthesis of collagen, elastin.
Literature
    required literature
  • RODWELL, Victor W., David A. BENDER, Kathleen M. BOTHAM, Peter J. KENNELLY and P. Anthony WEIL. Harper's illustrated biochemistry. Thirty-first edition. New York: McGraw-Hill, 2018, x, 789. ISBN 9781260288421. info
    recommended literature
  • Essentials of Medical Chemistry and Biochemistry (Ed. Dostál J.). Download: https://portal.med.muni.cz/article-623-essentials-of-medical-chemistry-and-biochemistry.html
  • LIEBERMAN, Michael and Alisa PEET. Marks' basic medical biochemistry : a clinical approach. Illustrated by Matthew Chansky. 5th edition. Philadelphia: Wolters Kluwer, 2018, xii, 1051. ISBN 9781496387721. info
  • VASUDEVAN, D. M., S. SREEKUMARI and Kannan VAIDYANATHAN. Textbook of biochemistry for medical students. Eighth edition. New Delhi: Jaypee The Health Sciences Publisher, 2016, xvi, 718. ISBN 9789385999741. info
  • KOOLMAN, Jan and Klaus-Heinrich ROEHM. Color Atlas of Biochemistry. 3rd ed. Georg Thieme Verlag, 2013. ISBN 978-3-13-100373-7. info
Teaching methods
Lectures.
Assessment methods
Students pass to continuing education in the next (spring) semester without their knowledge being verified in the current (autumn) semester.
Language of instruction
English
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 45.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2024.
  • Enrolment Statistics (autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2023/aVLBC0321p