Bi3010 Electron microscopy

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
0/2/0. 3 credit(s). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
Teacher(s)
Mgr. Iveta Hodová, Ph.D. (lecturer)
Guaranteed by
Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Prerequisites (in Czech)
Bi1050 Biological techniques || Bi2302 Zoological microtechnique || Bi1301 Microscopic Techniques && ( Bi2000 Animal System and Evolution || Bi2030 System evol. higher plants || Bi2090 System evolution vertebrates || SOUHLAS)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 0/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe to the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain of the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledges to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.
Syllabus
  • The theoretical part:
  • an introduction to electron microscopy
  • transmission electron microscope
  • preparative methods for TEM
  • scanning electron microscope
  • preparative methods for SEM
  • electronmicroscopic cytology
  • The practical part:
  • preparing glass knives
  • preparing grids for ultrathin sections
  • cutting and staining of semi-thin sections
  • preparing samples for SEM
  • individual routine operation of the electron microscope
Literature
  • KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
  • MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info
Teaching methods
theoretical lectures, laboratory practice, individual routine operation of the electron microscope
Assessment methods
colloquium
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: pro studenty 2. a vyšších ročníků, studenti 1. ročníků jen v případě volných míst.
Information on course enrolment limitations: 6 skupin po 10 studentech
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Autumn 2002, Autumn 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.