C5990 Aplikovaná enzymologie

Faculty of Science
Autumn 2002
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Initial information on enzymes, basic definitions, history. 2. Enzyme aktivity, units, methods of measurement, suitable substrates. 3. Isolation of enzymes, purification procedures, characterization. 4. Mechanisms of enzyme catalysis. 5. Kinetics of enzyme reaction with substrate, Michaelis-Menten equation, kinetic parameters, vmax (vlim), Km a vmax / Km. 5. Multisubstrate reactions, classification and kinetics, determination of mechanism. 6. Inhibition, reversible and irreversible inhibitors, methods of evaluation. 7. Software for enzyme kinetics. EnzLab, Gepasi. 8. Effect of external factors on enzyme reactions, pH, temperature, viscosity. 9. Cooperation effects, allosteric enzymes, Hill equation. 10. Imobilized enzymes, enzyme reactors. 11. Bioanalytical applications of enzymes. 12. Application of enzymes in immunochemical techniques. 13. Industrial applications of enzymes.
Assessment methods (in Czech)
ústní zkouška nebo kolokvium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Autumn 2002, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2002/C5990