PřF:Bi5710c Microbiology - pr. - Course Information

Bi5710c Microbiology - practice

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Andrea Vávrová, Ph.D. (seminar tutor)
doc. Ing. Martin Krsek, CSc., MSc (seminar tutor)

Guaranteed by

doc. RNDr. Miroslav Němec, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Miroslav Němec, CSc.

Timetable of Seminar Groups

Bi5710c/01: Tue 17:00–18:50 Bpb,02012, M. Krsek, A. Vávrová
Bi5710c/02: Wed 13:00–14:50 Bpb,02012, M. Krsek, A. Vávrová
Bi5710c/03: Wed 15:00–16:50 Bpb,02012, M. Krsek, A. Vávrová
Bi5710c/04: Thu 12:00–13:50 Bpb,02012, M. Krsek, A. Vávrová
Bi5710c/05: Thu 16:00–17:50 Bpb,02012, M. Krsek, A. Vávrová
Bi5710c/06: Tue 15:00–16:50 Bpb,02012, M. Krsek, A. Vávrová

Prerequisites (in Czech)

NOW( Bi5710 Microbiology )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)
• Biochemistry (programme PřF, B-CH)
• Biochemistry (programme PřF, M-CH)
• Biochemistry (programme PřF, N-CH)

Course objectives

The aim of the practical course of general microbiology are basic methods used in microbiological laboratory (methods of sterile inoculation and cultivation, slides preparation and microscopic examination of microorganisms, determination of the number of bacteria in different samples).

Syllabus

• 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of microorganisms 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition) 5. Determination of number of bacteria by cultivation method 6. Bacteria in water - basic microbiological analysis of water 7. Determination of the concentration of antibiotics by microbiological methods. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - use of biochemical tests and standardized Microsystems 9. Yeasts, test of vitality 10. Microorganisms in soil. Inoculation of microscopic fungi. 11. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negativ - staining, capsule - staining. Microscopic examination of endospores. 12. Protocols, written test and identification of unknown bacterial strain by Gramm staining.

Literature

• JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996, 67 pp. ISBN 80-210-1374-5. info

Assessment methods

Active participation on practicals, submission of reports, written test and identification of an unknown bacterial strain by Gram staining.

Language of instruction

Czech

Follow-Up Courses

• Bi5420 General virology
• Bi6700 Taxonomy of prokaryotes
• Bi6721 Special Methods of Microorganisms Analysis
• Bi7330 Cytology and morphology of bacteria

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• Bi2060c Basic microbiology - practice
  NOW(Bi2060) && !NOWANY(Bi4091c, Bi5710c, B4090c) && !Bi4091c && !Bi5710c && !Bi4090c  
• Bi4090c General microbiology - practical course
  NOW(Bi4090) && !NOWANY(Bi4091c, Bi5710c, Bi2060c) && !Bi4091c && !Bi5710c && !Bi2060c && rocnik(2)  
• Bi4091c Practise from general microbiology
  NOW(Bi4090) && !NOWANY(Bi4090c, Bi5710c, Bi2060c) && !Bi4090c && !Bi5710c && !Bi2060c && (2)  
• Bi7031cen Microbiology – Practical Course
  (!Bi5710c) && (!Bi4090c) && (!Bi2060c) && !NOWANY(Bi5710c,Bi4090c,Bi2060c)  

Teacher's information

http://www.sci.muni.cz/mikrob/obecna.html

• Enrolment Statistics (Autumn 2008, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2008/Bi5710c