VLIM051c Immunology-practicals

Faculty of Medicine
Autumn 2006
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jindřich Lokaj, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Timetable of Seminar Groups
VLIM051c/01: Wed 9:30–11:10 ÚKIA N01021
VLIM051c/02: Wed 9:30–11:10 ÚKIA N01021
VLIM051c/03: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/04: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/05: Fri 9:30–11:10 ÚKIA N01021
VLIM051c/06: Fri 9:30–11:10 ÚKIA N01021
VLIM051c/07: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/08: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/09: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/15: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/16: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/17: Thu 9:30–11:10 ÚKIA N01021
VLIM051c/18: Thu 9:30–11:10 ÚKIA N01021
VLIM051c/19: Thu 7:30–9:10 ÚKIA N01021
VLIM051c/20: Thu 7:30–9:10 ÚKIA N01021
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med.Chem.- lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II - lecture && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical ethics
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused to clinical and laboratory aspects of immunology in medicine. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment). In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed. Also immunological problems associated with transplantation and malignancies will be dealt with. After finishing this course the student will understand the pathogenesis of immunopathological diseases and know the clinical manifestation and the treatment approaches to the most severe disturbances of the immune system.
Syllabus
  • Clinical immunology laboratory Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions. Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology. Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation. Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells. B lymphocytes and immunoglobulins. . Biological properties of antibodies. Primary and secondary immunodeficiency disorders. HIV disease. Active and passive immunisation. Immunoglobulin therapy. Immunostimulation. Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies. Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated. Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock. Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 1st ed. Brno: Masarykova univerzita, 2007, 59 pp. ISBN 978-80-210-4227-8. info
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 3. vyd. Praha: Triton, 2005, 279 s. ISBN 8072546864. info
Assessment methods
Givng of the credit is conditioned by active attendance of 90% of the pracical lessons.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (Autumn 2006, recent)
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