PřF:Bi1301 Microscopic Techniques - Course Information

Bi1301 Microscopic Methods for the Study of Plant Structure

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Hana Cempírková, Ph.D. (seminar tutor)
RNDr. Jaroslava Dubová, CSc. (seminar tutor)

Guaranteed by

RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi1301/01: Thu 10:00–11:50 C13/332, H. Cempírková
Bi1301/02: Thu 16:00–17:50 C13/332, J. Dubová

Prerequisites (in Czech)

(! Bi1050 Biological techniques ) && (!NOW( Bi1050 Biological techniques ))

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of this practical course students should be able to:
describe the development of the light microscope and actual construction of the light microscope;
operate the light microscopes in different modes and techniques;
use various methods for preparations from botanical objects;
document these objects by means of microphotography;
process the microphotographs.

Syllabus

• Introduction to the Methods for the Study of Plant Structure: Light Microscope Development, Structure and Function. Koehler Illumination.
• Replicas: Study of the Surface Structure by Light Microscope. Stoma. Subsidiary and Neighbour Cells; Stoma Types.
• Cleared Preparations: Angiosperm Embryo Development.
• Squash Methods: Cell Cycle, Mitosis, Chromosome Staining.
• Anatomical Methods: Fixation. Dehydration. Infiltration and Embedding. Paraffin Sections. Sections Staining and Mounting.
• Vital Preparations: Phase Contrast Microscopy, Nomarski DIC.
• Pollen: Differential Staining and Pollen Viability
• Fluorescence Microscopy: Primary and Secondary Fluorescence, Immunofluorescence.
• Documentation: Measurements. Photomicrography.

Literature

recommended literature
• HABROVÁ, Věra. Mikroskopická technika. Praha: SPN, 1990. info
• KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
• PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
• NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
• Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
• ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
• PAZOUREK, Jaroslav. Atlas anatomické stavby rostlin. Praha: Karolinum, 1992. info
• KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
• BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
• BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
• Plant cell biology : a practical approach. Edited by C. R. Hawes - Béatrice Satiat-Jeunemaitre. 2nd ed. Oxford: Oxford University Press, 2001, xx, 338. ISBN 0199638659. info
• Plant cell biology : a practical approach. Edited by C. R. Hawes - Béatrice Satiat-Jeunemaitre. 2nd ed. Oxford: Oxford University Press, 2001, xx, 338. ISBN 0199638659. info

Teaching methods

Presentations covering theoretical bases of different procedures for the study of plant tissue structures using the light microscope are used as an introduction of each lesson. Practical procedures of selected histological techniques lead to microscopic preparation which is analysed by means of proper method of the light microscopy. Plant structure is documented by drawing or as microphotography. Protocol of each procedure containing theoretical base as well as analysed results is prepared by each student as homework.

Assessment methods

Control of protocols of used methods, written test, conclusion.

Language of instruction

Czech

Follow-Up Courses

• Bi7270 Plant Embryology

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• Bi1050 Biological techniques
  !Bi2302 && !Bi1301 && (!NOW(Bi2302)) && (!NOW(Bi1301))  

• Enrolment Statistics (Autumn 2015, recent)
  
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