PřF:Bi1301 Microscopic Techniques - Course Information
Bi1301 Microscopic Methods for the Study of Plant Structure
Faculty of ScienceAutumn 2005
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (seminar tutor)
Ing. Martina Hájková (seminar tutor) - Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová - Timetable of Seminar Groups
- Bi1301/01: Mon 12:00–13:50 Bpr,02004, J. Dubová
Bi1301/02: Mon 14:00–15:50 Bpr,02004, J. Dubová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- General Biology (programme PřF, B-BI, specialization Plant Physiology)
- Systematic Biology and Ecology (programme PřF, B-BI, specialization Systematic Botany and Ecology)
- Course objectives
- Introduction to the Methods for the Study of Plant Structure: Light Microscope as a Tool. Replicas for the Study of Surface by Light Microscope. Whole Mount of Cleared Preparations. Squash Methods. Histology and Histochemistry. Interference Microscopy. Fluorescence Microscopy. Documentation.
- Syllabus
- Introduction to the Methods for the Study of Plant Structure: Light Microscope Development, Structure and Function. Koehler Illumination. Replicas: Study of the Surface Structure by Light Microscope. Stoma. Subsidiary cells. Cleared Preparations. Angiosperm Embryo Development. Squash Methods: Cell Cycle, Mitosis, Chromosome Staining. Anatomical Methods: Fixation. Dehydration. Infiltration and Embedding. Paraffin Sections. Staining and Mounting. Vital Preparations: Phase Contrast Microscopy, Nomarski DIC. Fluorescence Microscopy. DAPI staining. Documentation: Measurements. Photomicrography.
- Literature
- HABROVÁ, Věra. Mikroskopická technika. Praha: SPN, 1990. info
- KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- PAZOUREK, Jaroslav. Atlas anatomické stavby rostlin. Praha: Karolinum, 1992. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Bi1050 Biological techniques
!Bi2302 && !Bi1301 && (!NOW(Bi2302)) && (!NOW(Bi1301))
- Bi1050 Biological techniques
- Enrolment Statistics (Autumn 2005, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2005/Bi1301