Bi1301 Microscopic Methods for the Study of Plant Structure

Faculty of Science
Autumn 2007
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Jaroslava Dubová, CSc. (seminar tutor)
Guaranteed by
RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová
Timetable of Seminar Groups
Bi1301/01: Mon 8:00–9:50 BprR, J. Dubová
Bi1301/02: Fri 9:00–10:50 BprR, J. Dubová
Bi1301/03: Mon 10:00–11:50 BprR, J. Dubová
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introduction to the Methods for the Study of Plant Structure: Light Microscope as a Tool. Replicas for the Study of Surface by Light Microscope. Whole Mount of Cleared Preparations. Squash Methods. Histology and Histochemistry. Interference Microscopy. Fluorescence Microscopy. Documentation.
Syllabus
  • Introduction to the Methods for the Study of Plant Structure: Light Microscope Development, Structure and Function. Koehler Illumination. Replicas: Study of the Surface Structure by Light Microscope. Stoma. Subsidiary cells. Cleared Preparations. Angiosperm Embryo Development. Squash Methods: Cell Cycle, Mitosis, Chromosome Staining. Anatomical Methods: Fixation. Dehydration. Infiltration and Embedding. Paraffin Sections. Staining and Mounting. Vital Preparations: Phase Contrast Microscopy, Nomarski DIC. Fluorescence Microscopy. DAPI staining. Documentation: Measurements. Photomicrography.
Literature
  • HABROVÁ, Věra. Mikroskopická technika. Praha: SPN, 1990. info
  • KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
  • PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
  • NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
  • Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
  • ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
  • PAZOUREK, Jaroslav. Atlas anatomické stavby rostlin. Praha: Karolinum, 1992. info
  • KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2007, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2007/Bi1301