PřF:Bi1301 Microscopic Techniques - Course Information

Bi1301 Microscopic Methods for the Study of Plant Structure

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching

Teacher(s)

Mgr. Hana Cempírková, Ph.D. (seminar tutor)

Guaranteed by

Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi1301/01: Wed 12:00–13:50 D36/209, H. Cempírková
Bi1301/02: Wed 14:00–15:50 D36/209, H. Cempírková

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The main objectives of the course are as follows:
- Introduction to the principles of microscopy: understanding the basic principles of different types of microscopes;
- Mastering different microscopy techniques such as light microscopy, phase contrast microscopy, polarizing microscopy, etc.;
- Preparation of microscopic specimens: students will learn how to prepare specimens appropriately for microscopic observation and how to identify different types of plant cells, tissues and organs;
- Staining microscopic specimens: students will learn the different staining techniques used in staining plant material;
- Creating permanent slides: students will learn the procedures necessary to create permanent microscopic slides;
- Advantages and limitations of different methods: students will understand the advantages and limitations of different microscopy methods and when it is appropriate to use a particular technique;
- Applications in different disciplines: students will learn about applications of different methods in different fields of plant research.

Learning outcomes

At the end of this practical course students should be able to:
describe the development of the light microscope and actual construction of the light microscope;
operate the light microscopes in different modes and techniques;
use various methods for preparations from botanical objects;
document these objects by means of microphotography;
process the microphotographs.

Syllabus

• Introduction to the Methods for the Study of Plant Structure: Light Microscope Development, Structure and Function. Koehler Illumination.
• Replicas: Study of the Surface Structure by Light Microscope. Stoma. Subsidiary and Neighbour Cells; Stoma Types.
• Cleared Preparations: Angiosperm Embryo Development.
• Root tip squash Method: Cell Cycle, Mitosis, Chromosome Staining.
• Anatomical Methods: Fixation. Dehydration. Infiltration and Embedding. Paraffin Sections. Sections Staining and Mounting.
• Vital Preparations: Phase Contrast Microscopy, Nomarski DIC.
• Pollen: Differential Staining and Pollen Viability
• Fluorescence Microscopy: Primary and Secondary Fluorescence, Immunofluorescence.
• Documentation: Measurements. Photomicrography.

Literature

recommended literature
• Plant cell biology : a practical approach. Edited by C. R. Hawes - Béatrice Satiat-Jeunemaitre. 2nd ed. Oxford: Oxford University Press, 2001, xx, 338. ISBN 0199638659. info
• KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
• PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
• KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info

not specified
• Plant microtechniques and protocols. Edited by Edward Chee-Tak Yeung - Claudio Stasolla - Michael John Sumner. Cham: Springer, 2015, xiv, 576. ISBN 9783319199436. info
• LUX, Alexander, Milan BALÁŽ, Marie KUMMEROVÁ, Aleš SOUKUP, Olga VOTRUBOVÁ, Jun ABE, Shigenori MORITA and Thomas ROST. Obrazový průvodce anatomií rostlin - Visual Guide to Plant Anatomy (Visual Guide to Plant Anatomy). 1st ed. Praha: Academia, 2017, 325 pp. ISBN 978-80-200-2620-0. URL info
• RUZIN, Steven E. Plant microtechnique and microscopy. Oxford: Oxford University Press, 1999, xi, 322. ISBN 9780195089561. info

Teaching methods

Presentations covering theoretical bases of different procedures for the study of plant tissue structures using the light microscope are used as an introduction of each lesson. Practical procedures of selected histological techniques lead to microscopic preparation which is analysed by means of proper method of the light microscopy. Plant structure is documented by drawing or as microphotography. Protocol of each procedure containing theoretical base as well as analysed results is prepared by each student as homework.

Assessment methods

Checking of protocols of used methods, one written test.

Language of instruction

Czech

Follow-Up Courses

• Bi7270 Plant Embryology

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• Bi1050 Biological techniques
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