Bi2302 Zoology microtechnique

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: k (colloquium).
Teacher(s)
RNDr. Božena Koubková, Ph.D. (seminar tutor)
Guaranteed by
RNDr. Božena Koubková, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: RNDr. Božena Koubková, Ph.D.
Prerequisites (in Czech)
(! Bi1050 Biological techniques ) && (!NOW( Bi1050 Biological techniques ))
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, B-BI, specialization Physiology of Animals)
Course objectives
At the end of this course, students should be able to: work with light microscope, aware of the specialized microscopy techniques used in   zoology (dark field, phase contrast, differential interference contrast, fluorescence, confocal microscopy, measuring, photomicrograph, drawing), prepare basic types of microscope slides (fixation, staining, mounting in the media, histological slides).
Syllabus
  • Light microscopy - dark field, phase contrast, differential interference contrast, fluorescence, confocal microscopy, measurement methods, photomicrography, drawing;. Basic zoological techniques - nativ slides, vital staining (neutral red, Kongo red, Methylen green, Janus green B). Permanent slides, framing, fixation, Carmine and hematoxylin staining; glycerol, glycerol jelly, Liquido-Faure, PVA; resinous media - canada balsam. Histological slides - embedding, cutting and staining.
Literature
  • Handbook of biological confocal microscopy. Edited by James B. Pawley. 3rd ed. New York: Springer, 2006, xxviii, 98. ISBN 038725921X. info
  • MURPHY, Douglas B. Fundamentals of light microscopy and electronic imaging. New York: Wiley-Liss, 2001, xii, 368 s. ISBN 0-471-25391-X. info
  • KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
Teaching methods
laboratory practice
Assessment methods
Lectures and laboratory practice, written test, colloquium Final written test usually consists of 20 questions rated 20 points. To successfully manage the need to reach at least 15 points.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Autumn 2002, Spring 2003, Autumn 2003, Spring 2004, Autumn 2004, Spring 2005, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Spring 2008, Autumn 2008, Spring 2009, Autumn 2009, Spring 2010, Autumn 2010, Spring 2011, Autumn 2011, Spring 2012, Autumn 2011 - acreditation, spring 2012 - acreditation, Autumn 2012, Spring 2013, Autumn 2013, Spring 2014, Autumn 2014, Spring 2015, Autumn 2015, Spring 2016, Autumn 2016, Spring 2017, autumn 2017, spring 2018, Autumn 2018, Spring 2019, Autumn 2019, Spring 2020, Autumn 2020, Spring 2021, autumn 2021, Autumn 2022, Spring 2023, Autumn 2023, Spring 2024, Autumn 2024, Spring 2025.