PřF:Bi2302 Zoology microtechnique - Course Information
Bi2302 Zoology microtechnique
Faculty of ScienceAutumn 2012
- Extent and Intensity
- 0/2/0. 3 credit(s). Type of Completion: k (colloquium).
- Teacher(s)
- RNDr. Božena Koubková, Ph.D. (seminar tutor)
- Guaranteed by
- RNDr. Božena Koubková, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: RNDr. Božena Koubková, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi2302/01: Wed 9:00–10:50 BpeR, B. Koubková
Bi2302/02: Wed 11:00–12:50 BpeR, B. Koubková
Bi2302/03: Wed 13:00–14:50 BpeR, B. Koubková
Bi2302/04: Wed 15:00–16:50 BpeR, B. Koubková - Prerequisites (in Czech)
- (! Bi1050 Biological techniques ) && (!NOW( Bi1050 Biological techniques ))
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, B-BI, specialization Physiology of Animals)
- Special Biology (programme PřF, B-EXB)
- Special Biology (programme PřF, B-EXB, specialization Experimental Biology of Animals and Immunology)
- Course objectives
- At the end of this course, students should be able to: work with light microscope, aware of the specialized microscopy techniques used in zoology (dark field, phase contrast, differential interference contrast, fluorescence, confocal microscopy, measuring, photomicrograph, drawing), prepare basic types of microscope slides (fixation, staining, mounting in the media, histological slides).
- Syllabus
- Light microscopy - dark field, phase contrast, differential interference contrast, fluorescence, confocal microscopy, measurement methods, photomicrography, drawing;. Basic zoological techniques - nativ slides, vital staining (neutral red, Kongo red, Methylen green, Janus green B). Permanent slides, framing, fixation, Carmine and hematoxylin staining; glycerol, glycerol jelly, Liquido-Faure, PVA; resinous media - canada balsam. Histological slides - embedding, cutting and staining.
- Literature
- Handbook of biological confocal microscopy. Edited by James B. Pawley. 3rd ed. New York: Springer, 2006, xxviii, 98. ISBN 038725921X. info
- MURPHY, Douglas B. Fundamentals of light microscopy and electronic imaging. New York: Wiley-Liss, 2001, xii, 368 s. ISBN 0-471-25391-X. info
- KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
- Teaching methods
- laboratory practice
- Assessment methods
- Lectures and laboratory practice, written test, colloquium Final written test usually consists of 20 questions rated 20 points. To successfully manage the need to reach at least 15 points.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi1050 Biological techniques
!Bi2302 && !Bi1301 && (!NOW(Bi2302)) && (!NOW(Bi1301)) - Bi4170 Optical contrast and imaging methods
Bi1050||Bi2302||Bi1301
- Bi1050 Biological techniques
- Enrolment Statistics (Autumn 2012, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2012/Bi2302