PřF:Bi2302 Zoological microtechnique - Course Information
Bi2302 Zoological microtechnique .
Faculty of ScienceSpring 2013
- Extent and Intensity
- 0/2/0. 3 credit(s). Type of Completion: k (colloquium).
- Teacher(s)
- Mgr. Veronika Konstanzová, Ph.D. (seminar tutor)
RNDr. Božena Koubková, Ph.D. (seminar tutor) - Guaranteed by
- RNDr. Božena Koubková, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: RNDr. Božena Koubková, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi2302/01: Tue 10:00–11:50 BpeR, B. Koubková
Bi2302/02: Tue 12:00–13:50 BpeR, B. Koubková
Bi2302/03: Tue 14:00–15:50 BpeR, V. Konstanzová
Bi2302/04: Thu 11:00–12:50 BpeR, B. Koubková
Bi2302/05: Thu 13:00–14:50 BpeR, B. Koubková
Bi2302/06: Thu 15:00–16:50 BpeR, B. Koubková - Prerequisites (in Czech)
- (! Bi1050 Biological techniques ) && (!NOW( Bi1050 Biological techniques ))
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- At the end of this course, students should be able to: work with light microscope, aware of the specialized microscopy techniques used in zoology (dark field, phase contrast, differential interference contrast, fluorescence, confocal microscopy, measuring, photomicrograph, drawing), prepare basic types of microscope slides (fixation, staining, mounting in the media, histological slides).
- Syllabus
- Light microscopy - dark field, phase contrast, differential interference contrast, fluorescence, confocal microscopy, measurement methods, photomicrography, drawing;. Basic zoological techniques - nativ slides, vital staining (neutral red, Kongo red, Methylen green, Janus green B). Permanent slides, framing, fixation, Carmine and hematoxylin staining; glycerol, glycerol jelly, Liquido-Faure, PVA; resinous media - canada balsam. Histological slides - embedding, cutting and staining.
- Literature
- Handbook of biological confocal microscopy. Edited by James B. Pawley. 3rd ed. New York: Springer, 2006, xxviii, 98. ISBN 038725921X. info
- MURPHY, Douglas B. Fundamentals of light microscopy and electronic imaging. New York: Wiley-Liss, 2001, xii, 368 s. ISBN 0-471-25391-X. info
- KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
- Teaching methods
- laboratory practice
- Assessment methods
- Lectures and practical exercises, written test, colloquium Lectures and laboratory practice, written test, colloquium Final written test usually consists of 20 questions rated 20 points. To successfully manage the need to reach at least 15 points.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
Information on completion of the course: pro udělení kolokvia je nutná docházka, protokoly, test
The course is taught each semester. - Listed among pre-requisites of other courses
- Bi1050 Biological techniques
!Bi2302 && !Bi1301 && (!NOW(Bi2302)) && (!NOW(Bi1301)) - Bi4170 Optical contrast and imaging methods
Bi1050||Bi2302||Bi1301
- Bi1050 Biological techniques
- Enrolment Statistics (Spring 2013, recent)
- Permalink: https://is.muni.cz/course/sci/spring2013/Bi2302