PřF:Bi2302 Zoological microtechnique - Course Information
Bi2302 Zoological microtechnique .
Faculty of ScienceSpring 2021
- Extent and Intensity
- 0/2/0. 3 credit(s). Type of Completion: k (colloquium).
- Teacher(s)
- Mgr. Mária Seifertová, Ph.D. (seminar tutor)
- Guaranteed by
- Mgr. Mária Seifertová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Mária Seifertová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science - Timetable
- Mon 31. 5. 8:00–12:00 D36/223, 13:00–17:00 D36/223, Tue 1. 6. 8:00–12:00 D36/223
- Prerequisites
- (! Bi1050 Biological techniques ) && (!NOW( Bi1050 Biological techniques ))
(!Bi1050) && (!NOW(Bi1050)) - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Medical Genetics and Molecular Diagnostics (programme PřF, B-LGM)
- Course objectives
- The main purpose in the course is to acquaint students with the basic techniques of light microscopy and the possibilities of processing biological material in the form of different types of prepared zoological microscope slides.
- Learning outcomes
- At the end of this course, students should be able to: work with light microscope; effectively use the specialized techniques of light microscopy (dark field, phase contrast, differential interference contrast, fluorescence, drawing, measuring); prepare basic types of microscope slides (fixation, staining, mounting in the media, histological slides) and propose a suitable method of processing biological material in the form of a prepared microscope slides.
- Syllabus
- Light microscopy - dark field, phase contrast, differential interference contrast, fluorescence, measurement methods, drawing. Basic zoological techniques - native slides, vital staining (neutral red, Methylene green); Permanent slides, framing, fixation, staining; glycerol, glycerol jelly, Liquido-Faure; resinous media - canada balsam, Entellan. Histological slides - embedding, cutting and staining.
- Literature
- Handbook of biological confocal microscopy. Edited by James B. Pawley. 3rd ed. New York: Springer, 2006, xxviii, 98. ISBN 038725921X. info
- MURPHY, Douglas B. Fundamentals of light microscopy and electronic imaging. New York: Wiley-Liss, 2001, xii, 368 s. ISBN 0-471-25391-X. info
- KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
- Teaching methods
- laboratory practice
- Assessment methods
- Lectures and laboratory practice, written test, colloquium Final written test usually consists of 20 questions rated 20 points. To successfully manage the need to reach at least 15 points.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Information on completion of the course: pro udělení zápočtu je nutné absolvovat test s úspěšností 75%
The course is taught each semester.
General note: předmět je ukončen kolokviem, jiná možnost ukončení není. - Listed among pre-requisites of other courses
- Bi1050 Biological techniques
!Bi2302 && !Bi1301 && (!NOW(Bi2302)) && (!NOW(Bi1301)) - Bi4170 Optical contrast and imaging methods
Bi1050||Bi2302||Bi1301
- Bi1050 Biological techniques
- Enrolment Statistics (Spring 2021, recent)
- Permalink: https://is.muni.cz/course/sci/spring2021/Bi2302