Bi2302 Zoological microtechnique .

Faculty of Science
Spring 2023
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: k (colloquium).
Teacher(s)
Mgr. Mária Seifertová, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Mária Seifertová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Mária Seifertová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science
Timetable
Wed 14:00–15:50 D36/223
Prerequisites
(! Bi1050 Biological techniques ) && (!NOW( Bi1050 Biological techniques ))
(!Bi1050) && (!NOW(Bi1050))
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main purpose in the course is to acquaint students with the basic techniques of light microscopy and the possibilities of processing biological material in the form of different types of prepared zoological microscope slides.
Learning outcomes
At the end of this course, students should be able to: work with light microscope; effectively use the specialized techniques of light microscopy (dark field, phase contrast, differential interference contrast, fluorescence, drawing, measuring); prepare basic types of microscope slides (fixation, staining, mounting in the media, histological slides) and propose a suitable method of processing biological material in the form of a prepared microscope slides.
Syllabus
  • Light microscopy - dark field, phase contrast, differential interference contrast, fluorescence, measurement methods, drawing. Basic zoological techniques - native slides, vital staining (neutral red, Methylene green); Permanent slides, framing, fixation, staining; glycerol, glycerol jelly, Liquido-Faure; resinous media - canada balsam, Entellan. Histological slides - embedding, cutting and staining.
Literature
  • Handbook of biological confocal microscopy. Edited by James B. Pawley. 3rd ed. New York: Springer, 2006, xxviii, 98. ISBN 038725921X. info
  • MURPHY, Douglas B. Fundamentals of light microscopy and electronic imaging. New York: Wiley-Liss, 2001, xii, 368 s. ISBN 0-471-25391-X. info
  • KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
Teaching methods
laboratory practice
Assessment methods
Lectures and laboratory practice, written test, colloquium Final written test usually consists of 20 questions rated 20 points. To successfully manage the need to reach at least 15 points.
Language of instruction
Czech
Further comments (probably available only in Czech)
Information on completion of the course: pro udělení zápočtu je nutné absolvovat test s úspěšností 75%
The course is taught each semester.
Information on the per-term frequency of the course: Předmět je ve stejné podobě vyučován v podzimním i jarním semestru.
General note: předmět je ukončen kolokviem, jiná možnost ukončení není.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Spring 2003, Autumn 2003, Spring 2004, Autumn 2004, Spring 2005, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Spring 2008, Autumn 2008, Spring 2009, Autumn 2009, Spring 2010, Autumn 2010, Spring 2011, Autumn 2011, Spring 2012, Autumn 2011 - acreditation, spring 2012 - acreditation, Autumn 2012, Spring 2013, Autumn 2013, Spring 2014, Autumn 2014, Spring 2015, Autumn 2015, Spring 2016, Autumn 2016, Spring 2017, autumn 2017, spring 2018, Autumn 2018, Spring 2019, Autumn 2019, Spring 2020, Autumn 2020, Spring 2021, autumn 2021, Autumn 2022, Autumn 2023, Spring 2024, Autumn 2024, Spring 2025.
  • Enrolment Statistics (Spring 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2023/Bi2302